Which is the best linker for fusion protein?

I want to fuse a protein (830 aa) with RFP. I have some linker sequences, but I'm not sure which linker is suitable? short or long? which sequence? this protein is a heat shock one and expresses...

05 June 2016 3,215 4 View

Why high ROS signal in flowcytometry and fluorescence microscope?

Hello I want to investigate the effect of high temperature on ROS production in yeast. I have yeast cells which grow at 30 degree as a control and experiment cells grown at high temperature.I used...

03 April 2016 6,374 6 View

Can anyone assist me in avoiding primer dimer in real-time PCR?

Hi. I did real-time PCR for 50% samples without any problem. I had just one great pick for my gene. No primer dimer. but suddenly, for other 50% samples I got primer dimer pick as well as my gene...

31 December 2015 4,397 7 View

Serum starvation in cell line treatment?

How long after seeding of cell line, should I do serum starvation or serum 4%? 24 h or less? How long should it last (before treatment) or serum 4%? 24 h or less? i i want to do serum starvation...

11 December 2014 3,961 3 View

Which cell line of C6, B65 and B92 is appropriate for study of memory gene expression after treatment with a drug?

I have to select one of them (no other cell lines), because my study is about rats and there are only these cell lines of brain in my country.

08 September 2014 5,149 1 View

Which neuronal cell line is a appropriate selection?

Hi. I want to investigate the effect of an herbal drug for memory genes. I selected PC12 cell line. This cell line differentiates neuron-like cells after NGF treatment. Because the NGF is very...

06 July 2014 9,922 4 View

Any suggested solvents for drug dissolution?

I want to treat my PC12 cell line with one drug, but the drug is insoluble in water and DMSO. I dissolved the drug in DMSO and media, but crystal was formed. What solvent do you suggest? What...

01 February 2014 4,384 4 View

Problems with PC12 cell culture?

Hi. I am working with a PC12 cell line. Most of cells in T-25 flask are round, dark green. What does this mean? Have my cells died or are in shock? How should I work with them?

31 December 2013 4,004 1 View

How to extract brucella DNA from human serum?

I want to extract brucella DNA from human serum. I have selected the "salting out" procedure, but I don't have a protocol for this. Could you suggest another procedure?

31 December 2013 8,739 4 View

I want to extract brucella DNA from human serum. I selected the "salting out" procedure, but I don't have a protocol for serum.

Could you suggest another procedure?

01 February 2013 6,124 1 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

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02 March 2021 3,060 3 View

Should we change media after1st 6hrs after plating HUVEC bought from INVITROGEN?it's not recomended by invitrogen.Will DMSO present in stock matter?

We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...

02 March 2021 3,713 2 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View