Dear Dr. Hanieh Rezaee

You should use the coated surface for cell attachment. The adhesion and proliferation of cystic fibrosis human bronchial epithelial cells (CFBE) cells is better promoted using different growth supports/matrix such as culture vessel coated with ECM molecules like collagen, laminin, etc., better mimicking the composition of basement membrane, which supports the cells in invivo setting.

You may carry out collagen coating of the culture vessel as follows.

Culture vessel may be coated for 1 h at room temperature with 1% (vol/vol) solution of a 3 mg/ml bovine collagen solution in PBS, then washed with distilled water and air-dried.

Regards,

Malcolm Nobre

thank you for your reply, but the coating media would affect on my western blot analysis as it composed of fibronectin