How can we choose the right method for Gaussian calculations?

I'm not working on biomolecules, I work on Organic, Organometallic, and sometimes inorganic structures. Do you know a simple way to apply the right calculation method? Is there any article or...

10 December 2020 3,396 5 View

How to dissolve sodium hyaluronate, 95% in water?

Hi, I am trying to dissolve Sodium hyaluronate, 95% in water with a concentration of 2.7% (w/v). However, it gets gel instead. So far, I have tried elevated temperature water bath (around 40...

30 August 2020 8,428 3 View

How we can find the extinction coefficient of antibodies?

I need extinction coefficient of anti-HER-2 antibody. where can I find it?

08 August 2019 9,276 1 View

I need a source about the different extraction methods and their effect on extraction of constituents in medicinal plants?

For example which method can extract polar and nonpolar constituents from medicinal plants easily?

07 May 2019 8,242 14 View

I have ordered some peptides and I need to add 5(6)-FAM to them. Anybody has any idea how i can do this?

These peptides are not on resin anymore and i do have Wang resin to maybe conjugate them back on the resin before attaching the dye but i am not quite sure how to do so! Is there a better way to...

11 March 2019 547 9 View

Sequence without ATGG?

24 August 2016 5,615 3 View

Why my breast cancer cell lines started to grow slowly and detach?

Does anyone has advices or explanation for the following? I have three cells line MCF-7, T47D and MDA231, when I received these cells in 25 cm flasks from friend's laboratory, they were adherent...

09 May 2014 7,125 11 View

DNA extraction of helico form biopsy

I faced with problem about extracting DNA of H.pylori directly form biopsy samples I am not sure about my method I am using CTAB-Chlrophorm method but i didnt get any result yet could you help...

16 July 2010 8,782 1 View

Is there anybody ,who have synthesized silica nanoparticles ?

for synthesizing silver-silica nanoparticles by sol-gel method , i have probem with it .

01 January 1970 3,708 8 View

I need to image mesoporous silica nanoparticles that are in ~100 nm size and do high resolution TEM to look at mesopores. what grids should I use?

Need to image mesoporous silica nanoparticles using the TEM. Also, need high resolution TEM images to see the mesoporous structure. Kindly suggest what kind of grids to use. Thanks, Shatadru

02 March 2021 1,787 2 View

What pH condition required to coat APTES on silica coated Fe3O4?

It's a hydrolysis reaction, which may produce by either acid or base-catalyzed reaction. so can anybody help me with this, which condition is suitable for this reaction?

26 February 2021 1,760 8 View

My silica gel column dried can I somehoe save the trapped matter?

I tried to separate my substance from a protein in a sillica gel column. By mistake, it dried and cracked. Can I save the trapped substance? Can I repack it ,or at least elute all of the trapped...

22 February 2021 1,789 3 View

Amorphous silica XRD peak at around 10 degree?

21 February 2021 3,417 5 View

Reaction condition to convert NH2 to COOH?

I need to couple Dopamine by NH2 end to silica nanoparticles coated by PEG-NH2, but first i need to convert the PEG-NH2 to COOH by using succinic anhydrous ... can you suggest a reaction condition ?

13 February 2021 2,768 4 View

Extraction of DNA from insect samples conserved in silica gel?

Hi everybody, can anybody suggest how to extract DNA from insect samples conserved in silica gel (in my case, mosquitoes)? Does anybody know a commericial kit which does that or a particular...

10 February 2021 3,392 4 View

What is the type or grade of ethanol used in SOL-GEL?

I'm preparing materials that will be use in SOL-GEL to produce silica gel, I will use Tetraethyl orthosilicate (TEOS) as precursor, and ethanol as solvent , but when I search to buy ethanol, the...

01 February 2021 5,153 4 View

What is the most efficient way to generate amorphous silica in LAMMPS?

I would like to generate multiple large samples of amorphous silica in LAMMPS. Currently I am generating it by beginning with a lattice of silicon and oxygen atoms and using a melting and...

28 January 2021 6,128 4 View

How best can one separate n-hexane-soluble fraction of plant extract that binds strongly to silica gel columns and what are the possible compounds?

I wanted to elute n-hexane-soluble fraction of a plant extract in a silica gel column using different proportions of dichloromethane/n-hexane. The bands separated visibly initially but moved only...

18 January 2021 9,270 3 View

Is there any special rule to find out possible room-temperature stable silicates' chemical composition, if not crystal structure itself?

Suppose one needs to find out room-temperature stable silicates chemical composition of a particular cation, suppose magnesium. Is there any rule to estimate which stoichiometric values of metal...

13 January 2021 7,385 4 View