Hanieh Rezaee

3 Questions 4 Answers 0 Followers

Questions related from Hanieh Rezaee

Dose any one use PCC1 plasmid?

I insert the 4kb DNA in to PCC1 plasmid. but I have a problem with transformation into top10 component bacteria plasmid is resistant to chloramphenicol and I add 0.5mg/ml in Lb media protocol...

15 October 2017 4,125 2 View

Sequence without ATGG?

Hello I am cloning nanog in pCDNA vector but after ATG I dont have G for kozak sequence can I add just ccacc before ATG can you help me?

25 August 2016 5,699 3 View

DNA extraction of helico form biopsy

I faced with problem about extracting DNA of H.pylori directly form biopsy samples I am not sure about my method I am using CTAB-Chlrophorm method but i didnt get any result yet could you help...

17 July 2010 8,854 1 View