Cell pellet becomes sticky / insoluble in nuclear extraction buffer?

24 May 2016 8,013 2 View

Cell pellet becomes sticky / insoluble in nuclear extraction buffer?

04 May 2016 5,180 3 View

The meaning of X and the preparetion of X solution?

02 March 2016 2,506 2 View

Why does a stacking gel leak?

After I pouring resolving gel, I put a layer of isopropanol. Resolving gel looked polymerised well. However, after I pouring stacking gel on top of it and letting it to be polymerised, I found...

03 January 2016 3,368 7 View

Why does a stacking gel leak?

After I pouring resolving gel, I put a layer of isopropanol. Resolving gel looked polymerised well. However, after I pouring stacking gel on top of it and letting it to be polymerised, I found...

31 December 2015 7,316 7 View

How do I guarantee the number of cells for western blot or other experiments?

When I read through the method of some journals, they will mention the number of cells they used, for example 1x10^6 cells for immunoblotting. My question is how do we ensure that there is 1x10^6...

16 December 2015 2,621 4 View

Why does ethanol not affect the absorbance of Bradford assay?

11 December 2015 5,338 7 View

Spit ratio for cell tissue culture?

An old and stupid questions for all the newer of cell culture.. I found that there are two definitions of split ratio on the web: 1. 1:7 = 1ml of cell resuspended + 9ml of medium 2. 1:7 = 1ml from...

11 December 2015 8,287 5 View

How do I guarantee the number of cells for western blot or other experiments?

When I read through the method of some journals, they will mention the number of cells they used, for example 1x10^6 cells for immunoblotting. My question is how do we ensure that there is 1x10^6...

11 December 2015 6,390 5 View

What happens on my western blot membrane?

Refer to the picture attached, it shows some of the lanes doesnt show a nice band. May I know any possible reasons or causes that make this happen?

11 December 2015 3,023 6 View

Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

What are some important techniques for protein induction and SDS-PAGE analysis?

Hello everyone. I am fairly new in protein chemistry field. I am trying to perform a protein induction hoping to purify my protein of interest using FPLC method. However, I am having trouble on...

03 March 2021 8,237 3 View

SDS page software for analysis?

I did sds page to determine the difference in protein expression between 3 types of vaccine , but i don't have scanner or densitometer available. . so i wonder if there is a software to analyze...

02 March 2021 6,270 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

How can I represent SDS-PAGE results for machine learning analysis?

I am trying to classify and analyze the results of an SDS-PAGE based array for bacterial detection using machine learning, but I have trouble finding the best way to represent the results with...

27 February 2021 9,176 3 View

What is the most suitable resuspension buffer for TCA/acetone precipitated protein?

I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...

26 February 2021 7,129 3 View

Can anyone share his experience regards Smoothing in origin?

I am working on TiO2 composite material and the results of XRD have a lot of small/junk peaks and while I apply a smooth filter in Origin then the other characteristics peaks disappear so anyone...

26 February 2021 8,183 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View