We usually prepare concentrated (10X, 6X, or 4X) solution. How to determine the X of a solution? For example, how do we prepare 2X, 4X, and 6X SDS sample buffer?
A normal working solution is a 1x, or normal strength solution. We typically refer to the strength of a stock solution by a number followed by the times symbol x. For example, a stock solution that is concentrated by a factor of 10 is called a 10 times concentrated stock, a 10x concentrate, a solution of 10x strength, or simply a 10x solution.
Thus, for making 2x, 4x or 6x SDS sample buffer, we need to know how to make 1x SDS buffer,
1x SDS buffer needed: 28.8 g glycine, 6.04 g Tris base, 2 g SDS and make final volume to 2 L with ddH2O. so for making 2x buffer, just take twice amount of the all reagents and make the final volume to 2 L.
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