How can we stain d tissue section by mallory triple stain?

How can we stain d tissue section by mallory triple stain? If any one has the protocol of mallory triple stain then please help.

08 September 2018 7,550 1 View

Can we use phosphate buffer pH 8 for different parameters of MDA , reduced gultathione ,Catalase , SOD?

Can we use phosphate buffer pH 8 for tissue homogenate formation for different parameters of MDA ,reduced gultathione ,Catalase , SOD?

06 July 2017 3,532 13 View

Anyone has the standardized protocol for prepration of dose for rat by powdered curcumin ?

04 May 2017 1,874 2 View

How can we prepare homogente from tissue for total cholestrol estimation?

how can we prepare homogente from tissue for total cholestrol estimation?

02 March 2017 982 1 View

How to calculate the specific activity of MDA by wilbur method ?

how to calculate the specific activity of MDA by wilbur method ?

09 October 2016 6,234 1 View

How to calculate the specific activity of GPx by rotruck method ?

how to calculate the specific activity of GPx by rotruck method ?

09 October 2016 2,003 2 View

How can we prepare sodium carbonate buffer of 0.05 M from sodium carbonate and soidium bicarbonate?

how can we prepare sodium carbonate buffer of 0.05 M from sodium carbonate and soidium bicarbonate?

08 September 2016 3,234 1 View

What is the differnce between competitive RT- PCR and real time PCR?

what is the differnce between competitive RT- PCR and real time PCR?

07 August 2016 4,190 0 View

Can anyone give the protocol of giemsa satining of tissue ?

can anyone give the protocol of giemsa satining of tissue ?

07 August 2016 6,001 5 View

Can anyone give the protocol of giemsa satining of tissue after potasium dichromate fixation?

Can anyone give the protocol of giemsa satining of tissue after potasium dichromate fixation?

07 August 2016 4,045 0 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Genome-wide gene identification protocol ?

Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?

28 February 2021 8,095 1 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

How to add missing citation?

Dear colleagues, I have found that many authors have a concern regarding their missing citation in RG because often citation in RG is lower than google scholars. I also missed some citation...

26 February 2021 928 6 View

Centrality measures computation in Biblioshiny/ Vosviewer?

26 February 2021 7,279 2 View

What are the pros and cons for mice versus rats?

Hi Everyone, I am designing a pilot study using rodents to assess the effects of simulated space environments (radiation + altered gravity) on microbiome and immune system. I have limited...

24 February 2021 1,825 3 View

RNA-seq DEGs downstream analysis and reporting in literature?

I used EdgeR to get the list of DEGs (differentially expressed genes) from my liver RNA-seq experiment. I have done the gene overrepresentation and GSEA based pathway analysis. I am currently...

23 February 2021 8,969 1 View

Could you please suggests a tutorial or paper or any other materials to learn multiple gene expression in TCGA and GTEx databases?

I would like to analyze multiple gene expressions in normal and cancer tissue by using the data in the TCGA and GTEx databases. Please suggest a tutorial or paper showing step by step analysis of...

23 February 2021 9,007 3 View

What should be the duration of exposure to simulated space environments with Rodents?

Hi, I am currently working on a pilot study to get some data related to the impact of long duration spaceflight to the microbiome and the immune system. I plan to use rats and simulate...

22 February 2021 592 1 View

Are there any databases for gene co-expression or expression pattern clustering?

I am currently working on gene clustering based on co-expression pattern in mouse brain. The problem is I do not have some solid way to test my result. Are there any suggestions for databases...

22 February 2021 4,374 3 View