I think the problem is upper gel specially the wells.

Try to make it better and when you want to running the gel fill empty wells with loading buffer.

Thank you. So my last band line looks like moumtain. That reason is empty wells. Right?

maybe.

empty wells are cause of distributing among running.

check it first and if didnt work try other things, because your ladder run nice.

Thank you for your answer. I will try.

Bacteriocins are usually highly basic peptides with low molecular mass. Tris glycine gels may not be the best approach. I would try tricine gels.

http://depts.washington.edu/taneli/taneli/procedure/tris_tricinel.PDF