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Questions related from Woobin Hyun
Bacteriocin production is being optimized. However, pH, agitation rate, temperature and time all proceeded under the same conditions. In addition, the condition for growing the bacteria was that...
03 March 2019 3,663 5 View
Hello. I have some problem in SDS-PAGE. My sample condition was this. 1. Ammonium sulfate gradient(20~40%) 2. Dialysis(1kDa) 3. After filter then freezing dry. 4. Dissolve using phosphate...
10 October 2018 8,988 2 View
I did Tris-Glycine SDS-PAGE. I made this using the methods outlined in the molecular cloning book. but I couldn't see the straight band. What is a problem? I want to get straight band..
08 August 2018 7,171 5 View
Hello. I have some bacteriocin activity problem. My protocol is this. 1. I inoculated bacteria in broth. Then incubated overnight. 2. I inoculated 1% bacteria from bacteria broth(1). Then during...
05 May 2018 6,312 8 View
Hello. I am studying about bacteriocin. My cell-free supernatant(CFS) from bacillus subtilis can inhibit indicator strain. I want to know, inhibition factor in CFS is protein or peptide. So i...
05 May 2018 785 11 View
First, My lab bought anaerobic chamber. So we made perfectly anaerobic condition in anaerobic chamber. but anaerobic condition is steadily broken. How can I maintain anaerobic condition? My idea...
10 October 2017 7,683 3 View
My experiment is anaerobic bacterial bacteriophage isolation. But sometime anaerobic condition broken..(anaerobic chamber) if anaerobic condition broken, i always manual vacuum 20time. This is...
10 October 2017 9,960 2 View
