First, My lab bought anaerobic chamber. So we made perfectly anaerobic condition in anaerobic chamber. but anaerobic condition is steadily broken. How can I maintain anaerobic condition?

My idea is (1) pyrogallol, (2) Oxygen Scrubber( Is this permanent?)

(3) every 1or 2 week, 10 Time vacuum and insert N2

How can your lab maintain anaerobic condition?

(My experiment bacteria is Clostridium perfringens)

Please give me idea. Thank you.