Hello.

I have some bacteriocin activity problem.

My protocol is this.

1. I inoculated bacteria in broth. Then incubated overnight.

2. I inoculated 1% bacteria from bacteria broth(1). Then during 24hr, incubated.

3. 13500RPM, 5min centrifuge, and I used 0.2micro miter filter.

4. Finally, I did well diffusion assay.

My experiment result

First trial - 1.3cm

Second trial - 1.9cm

Third trial - 1.5cm

My result is very different... What is a problem? Is 1% inoculation problem?

More Woobin Hyun's questions See All
Similar questions and discussions