Hello.
I have some bacteriocin activity problem.
My protocol is this.
1. I inoculated bacteria in broth. Then incubated overnight.
2. I inoculated 1% bacteria from bacteria broth(1). Then during 24hr, incubated.
3. 13500RPM, 5min centrifuge, and I used 0.2micro miter filter.
4. Finally, I did well diffusion assay.
My experiment result
First trial - 1.3cm
Second trial - 1.9cm
Third trial - 1.5cm
My result is very different... What is a problem? Is 1% inoculation problem?