How can i remove oxygen in anaerobic chamber?

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Can you give us your opinion on optimizing bacteriocin production?

Bacteriocin production is being optimized. However, pH, agitation rate, temperature and time all proceeded under the same conditions. In addition, the condition for growing the bacteria was that...

02 March 2019 3,622 5 View

Why my band can not stain by coomassie blue R?

Hello. I have some problem in SDS-PAGE. My sample condition was this. 1. Ammonium sulfate gradient(20~40%) 2. Dialysis(1kDa) 3. After filter then freezing dry. 4. Dissolve using phosphate...

09 October 2018 8,956 2 View

Please can you tell me my Bacteriocin SDS-PAGE problem?

I did Tris-Glycine SDS-PAGE. I made this using the methods outlined in the molecular cloning book. but I couldn't see the straight band. What is a problem? I want to get straight band..

07 August 2018 7,127 5 View

Can you tell me bacteriocin enzyme treatment?

Hello. I am studying about bacteriocin. My cell-free supernatant(CFS) from bacillus subtilis can inhibit indicator strain. I want to know, inhibition factor in CFS is protein or peptide. So i...

04 May 2018 747 11 View

Can you tell me bacteriocin activity test problem in my protocol?

Hello. I have some bacteriocin activity problem. My protocol is this. 1. I inoculated bacteria in broth. Then incubated overnight. 2. I inoculated 1% bacteria from bacteria broth(1). Then during...

04 May 2018 6,256 8 View

How can i maintain anaerobic condition in anaerobic chamber?

First, My lab bought anaerobic chamber. So we made perfectly anaerobic condition in anaerobic chamber. but anaerobic condition is steadily broken. How can I maintain anaerobic condition? My idea...

09 October 2017 7,647 3 View

Does the pressure(-600 mmHg) affects pH?

When I conducted the degassing experiment using decompression under anaerobic condition(and I used ferrous based chelating agent), the oxidized ferric were observerd. So, could you tell me the...

03 March 2021 4,892 5 View

What is the best way to keep an anaerobic chamber anoxic in the absence of hydrogen?

I'm a graduate student who works with an anaerobe, so I often work in an anaerobic chamber. I've been working on an assay that involves the steady-state of the quinone/ quinol pool. Anoxic...

03 March 2021 2,971 1 View

Is there any rich, publicly available English literary corpus?

Hi everyone. I am looking for a rich corpus in English literature, a one which includes literary prose ( e.g., novels or short stories) . can anyone propose a good one? Best regards Fatemeh

01 March 2021 1,970 2 View

How to separate water from glycerol solvent in a solvent recovery vacuum distillation column?

I am currently doing my third year design project. I have been assigned to design a glycerol recovery column in vinyl acetate monomer production. The process unit before the recovery column is an...

01 March 2021 9,525 4 View

Which fluorescent dye to use for measuring total ROS production?

Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?

01 March 2021 9,447 3 View

Uncertianiny in using dry or wet basis in mixing a sediment with fuel oil?

Hello I'm currently working on a topic related to studying the anaerobic biodegradation of TPH in marine sediments. In this work, sediment should be mixed with fuel oil to a concentration of 20 g...

24 February 2021 9,839 3 View

Advice on growing lactobacillus species in the lab?

Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...

24 February 2021 3,157 6 View

Which database is the most suitable for checking English bigrams frequency?

Which database is the most suitable for checking English bigrams frequency? We want to assess the frequency of different English graphemes. Most of the databases that we have checked seem to...

18 February 2021 4,565 3 View

Small displacement measurement in vacuum?

Hello, I have a question. there is a circular thin foil which vibrates in vacuum, the foil restricted and moves up and down. it is like a diaphragm between two pipes . both sides are in vacuum but...

18 February 2021 8,075 3 View

Where would? can? positive charge(vacancy) be when grounded?

Hello I plasma(H2N2) treated Al2O3 plate (edge is connected to ground) and wondered if oxygen vacancy migration on the plate can occurs as electrons escape through edge. Or whatever positive...

15 February 2021 8,118 1 View