I need to perform PAGE for siRNA at different time points for serum stability with etbr.
Please share your protocol etc especially for using etbr post running the siRNAs.
Thanks in advance.
We performed the siRNA based knockdown assay with 10 different siRNAs and each siRNA with three different concentrations (0.1, 0.5, 1 nM) and repeated three times in triplicates (3-wells) each...
09 October 2017 3,509 4 View
Greetings...I am performing experiment of knockdown using siRNA with dual glo luciferase assay system E2920 (promega) and psi-check-TM2 plasmid1) what instrument should I use for two readings and...
04 May 2016 5,751 6 View
Showkat Ahmad How can we express the viral protein that is not complete ORF (i.e. with out start or stop codon) but a part of one complete gene and becomes functional protein after Post...
11 December 2015 6,205 4 View
I am Looking for a Science Journal with good impact factor and low publication cost to publish a review paper. Your suggestions would be appreciated.
06 August 2024 6,796 3 View
Currently, when I run SDS-PAGE, I don't see any bands at all, even though I used the same material just a day ago and it worked fine.... In our lab, we dilute the 10X running buffer to 1X and...
06 August 2024 5,373 2 View
I ran a SDS-page of a bacterial lysate and I want to quantify protein concentration in a specific band. I was thinking of using a standards ladder or make some standards are different...
05 August 2024 9,805 3 View
Any idea about 'International Research Journal of commerce , arts and science? Is it a UGC listed journal? Kindly advice
04 August 2024 7,367 3 View
As authors and academic writers, we usually prepare our manuscript using our own resources. We submit these manuscripts to scientific Journals for peer review and publication sometimes at a fee....
03 August 2024 7,304 2 View
We assume this to be true. Science leaves the era of mathematics and enters the era of matrix mechanics and the turning point is the discovery of numerical statistical theory called Cairo...
31 July 2024 3,900 2 View
Does anyone know of a module for the JAMOVI software that is capable of generating mean separations using the classic letters based on post hoc results (e.g., Tukey test)? If, as I believe, such...
31 July 2024 3,333 4 View
I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...
30 July 2024 6,420 1 View
In some data sources it has been grouped in Q1 and some shows it is Q2.
29 July 2024 4,227 2 View
I am using CuBr/THPTA for a click reaction in total cell lysates. I am facing issues with my protein sample in non-reducing SDS-PAGE where it's not migrating properly and most of it remains at the...
29 July 2024 950 4 View