Other than Ficoll- paque plus method, what are the other reagents that we can use for Isolation of Monocytes from blood??

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How to select drugs for repurposing?

Hello everyone, so I am searching for ways to find the method for picking the right drug for repurposing. This repurposing is for existing and new diseases. This is very important, please help.

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Any biomarker (cost effective) to detect gastric cancer?

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For sample preparation for GC-MS analysis is it compulsory to add internal standards..?

My sample is blood plasma.. If i need to do the GC-MS analysis to check the metabolites, is it necessary to add internal standards? Or can we do sample preparation without that??..

22 April 2024 3,217 3 View

At what temperature we should keep sucrose solution for sucrose gradient centrifugation?

For isolation of PBMC, at what temperature we should keep sucrose solution. Which method is better for PBMC isolation? Ficoll DGC method or Sucrose DGC method?

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How to obtain adolescent resilience questionnaire/scale/test for my thesis ?

I am doing my research on psychological resilience in adolescents, juvenile and non-juvenile, and for this purpose, I am searching for an resilience scale for data collection. please suggest to me...

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How to differentiate metal complex and metal organic framework?

Which characterization technique confirms the formation of metal organic frame work. My XRD pattern shows crystalline nature and the card number matches with literature. Is there any other...

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How to get wavefunction and density using Gaussian g09 ?

I want to calculate the density in the terms of basis functions.

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What is the difference between Ficoll and Ficoll paque plus?

Which is the most appropriate method for PBMC isolation from blood. What is the difference between ficoll and ficoll paque plus

18 September 2023 9,051 3 View

Audio script or vedio script difference ?

give 10 example of radio scrip and 10 example of TV serials

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Can I multiplex a mouse monoclonal and rat primary for IHC (mouse brain tissue)?

Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...

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Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

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Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

Interested in a SCOPUS collaboration?

Hi RG family. My team and I are working on some SCOPUS publications and we need co-authors who are willing and capable of undertaking both qualitative and quantitative-based studies. The scope...

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Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

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Is it common for heterotrophic bacteria to grow on Pro99 and SNAX media?

I am attempting to isolate picocyanobacteria from seawater (pre-filtered with a GF/D membrane) using the pour plate technique and filter plating method (Kearney et al., 2022) on Pro99 and SNAX...

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Addition of EDTA during the synthesis of copper nanoparticles to prevent it from being oxidized?

I've attempted to use this method to synthesize copper nanoparticles. Copper nanoparticles can be synthesised using a variety of precursor materials. CuSO4, distilled water, NaOH, and EDTA are...

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Question about MHB (Mycorrhiza Helper Bacteria) isolation technique??

What are the most effective isolation techniques for obtaining MHB?? Is there are best approach techniques? I keep struggling in obtaining it.

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