I want to calculate the density in the terms of basis functions.
i have issues in expressing a chicken protein in BL21 cells with his tag. I do not see induction even at 18 degrees. Any suggestions
15 February 2021 8,939 3 View
Dear friends i have only H&E stained slide and i have to do other special staining from this slide only. kindly suggest me the procedure for complete destaining of this slide.
13 December 2020 634 4 View
I am doing an experiment on proteins. I precipitated protein using TCA- acetone method and want to check purity on SDS-PAGE. I used 25-30 microgram protein for one well, after running SDS- PAGE,...
24 November 2020 8,885 3 View
Hi I want to create stable cell lines for membrane proteins of interest in inducible expression system. I wonder if anyone has used pTRE-tight-BI system to express two genes simultaneously in...
12 March 2020 2,832 3 View
Is it wise to use two different gaussians for two CVs during QM/MM metadynamics simulations?. Example for 0.5kcal/mol Gaussian height, For CV1 width is 0.3 and CV2 is 0.15. Where both CVs address...
10 March 2020 5,287 3 View
Is correlation test sufficient for proving the efficiency of the encryption scheme?
23 February 2020 3,524 4 View
I am working on silicon on insultor (SOI) on chip devices. I am struggling a lot to design a final layout of the waveguide device on both of the software (Ledit and Klayout software). These two...
16 September 2019 8,681 3 View
Whether increase or decrease in entropy of cipher image is good for encryption? How?Why?
06 May 2019 9,299 12 View
Sir i want extract all metadata from a digital audio file for authentication purpose.
16 February 2019 4,123 2 View
For doing PCR, instead of preparing the PCR mater mix solution each and every time, is it advisable to prepare it in a larger quantity and store it at -20C so that the PCR can be set up faster....
27 January 2019 7,112 19 View
When I run the job for TS(QST3) in gaseous phase, it was completed without any error. But when I add water in CPCM model it terminated with an error: Inconsistency: ModMin= 2 Eigenvalue=...
21 February 2021 3,198 2 View
Dear friends, I am a beginner at DFT calculation. I have done a structure optimization using DFT- B3LYP/6-31G*, Nevertheless, I don't know How to calculate the Atomic Polar Tensor (APT) in...
17 February 2021 9,082 3 View
Hello everyone, I want to perform some gaussian calculations and want to use Materials studio for my research work. Can anyone guide me how to get access to supercomputer resources in India for...
15 February 2021 8,264 3 View
Support vector machine
09 February 2021 6,405 3 View
DFT - gaussviev - gaussian09W - HOMO - LUMO
08 February 2021 6,012 5 View
I am trying to find methods for theoretical calculation of enthalpy of formation of Polymers. till now I have found the oligomeric method (Gaussian) which uses the extrapolation of results from...
05 February 2021 9,707 4 View
Dear all, I am trying to optimize a bimetallic complex using # opt=tight b3lyp/lanl2dz scf=qc geom=connectivity. The program is running for around 5 days but the size of the .chk remains same...
05 February 2021 8,721 3 View
I am doing optimization of a phytochemical but when i submitted the file for calculations it shows error 2070. Can anyone please tell me what does it mean? and what should i do to solve it. I have...
30 January 2021 7,932 1 View
In epidemiology, earthquakes, tokamak disruptions etc., there is possibility of approximations with the sequence of Gaussians and with the appropriate risks ( see my papers in Journal of Fusion...
26 January 2021 5,411 5 View
Hi all, Is there any software or webserver to read the molecular dynamics (MD) simulation trajectory which obtained from Ab initio MD simulation to calculate the atom-atom radial distribution...
25 January 2021 9,474 3 View
