Aparna Eledath Kolleseri

6 Questions 2 Answers 0 Followers

Questions related from Aparna Eledath Kolleseri

How to identify the metabolites from LC-HRMS peaks if there is no library provided?

I need to identify the metabolites in the blood. I have done LC-HRMS. since there is no library provided, I am finding difficulties identifying the hits. Any suggestions..?

20 December 2024 5,759 7 View

For sample preparation for GC-MS analysis is it compulsory to add internal standards..?

My sample is blood plasma.. If i need to do the GC-MS analysis to check the metabolites, is it necessary to add internal standards? Or can we do sample preparation without that??..

23 April 2024 3,262 3 View

At what temperature we should keep sucrose solution for sucrose gradient centrifugation?

For isolation of PBMC, at what temperature we should keep sucrose solution. Which method is better for PBMC isolation? Ficoll DGC method or Sucrose DGC method?

27 November 2023 3,766 2 View

Other than Ficoll- paque plus method, what are the other reagents that we can use for Isolation of Monocytes from blood??

I want to know what are the alternatives for Ficoll- paque plus. Can we use sucrose/percoll/Na metrizoate/Caesium chloride for Monocyte isolation

07 November 2023 5,631 2 View

What is the difference between Ficoll and Ficoll paque plus?

Which is the most appropriate method for PBMC isolation from blood. What is the difference between ficoll and ficoll paque plus

19 September 2023 9,089 3 View

I want to know the sample preparation methods for LC-MS/MS using blood sample. Can we convert liquid sample into solid or semisolid form?

To check the metabolites of blood , I want to use LC-MS/MS. So I want to know more about the sample preparation and is there any method to convert liquid sample into solid or semi solid form.

29 June 2023 7,138 3 View