Trouble shooting with his tagged protein expression in E.Coli?

i have issues in expressing a chicken protein in BL21 cells with his tag. I do not see induction even at 18 degrees. Any suggestions

15 February 2021 8,939 3 View

Two different gaussian width in QM/MM Metadynamics?

Is it wise to use two different gaussians for two CVs during QM/MM metadynamics simulations?. Example for 0.5kcal/mol Gaussian height, For CV1 width is 0.3 and CV2 is 0.15. Where both CVs address...

10 March 2020 5,287 3 View

What is the impact of correlation test in Image encryption ?

Is correlation test sufficient for proving the efficiency of the encryption scheme?

23 February 2020 3,524 4 View

What is the significance of image entropy ( plain image and cipher image) in image processing?

Whether increase or decrease in entropy of cipher image is good for encryption? How?Why?

06 May 2019 9,299 12 View

Can PCR master mix solution be prepared in advance and stored at -20C?

For doing PCR, instead of preparing the PCR mater mix solution each and every time, is it advisable to prepare it in a larger quantity and store it at -20C so that the PCR can be set up faster....

27 January 2019 7,112 19 View

What are the other options to play with audio files apart from shifting and transformation?

Any other specific operations with speech files?

23 September 2018 9,243 2 View

Can I stain cells with Annexin V after fixing with 70% ethanol?

I have treated cancer cells with inhibitors of cell cycle. In addition to looking at the stage of cell cycle they are in, I want to see if there is any apoptosis induced by the treatment. But I...

28 July 2018 1,242 3 View

How to measure the mesh size in COMSOL for PCF design for FEM analysis?

I want to know how to measure mesh size in COMSOL when are using initializing mesh than refined mode? Is that possible?

28 February 2018 4,418 3 View

Analytical formula of Natural Freqency of a composite with fixed-free (cantilever type) boundary condition?

I need to calculate the analytical relation for frequency of a cantilever composite beam, where one beam is prestressed on which an another non-prestressed beam is placed in a way to form the...

17 January 2018 2,773 9 View

What are the reasons for getting low value of R-square?

In my research I am getting a low value of R-square (0.253). What are the possible reasons for getting low value and How it can be interpreted?

16 September 2017 2,697 3 View

Resuspending cell pellet after ficoll centrifugation?

In my current project I am planning to isolate mononuclear cells from peritoneal ascites of cancer patients using ficoll centrifugation. However, following isolation of the mononuclear cell layer,...

26 October 2020 5,966 4 View

T Cell Isolation for RT-PCR?

I need a cell that will be HLA-DR positive on RT-PCR. For this, would it work if I directly isolate RNA from PBMC cells with Ficoll? Or should I necessarily culture these PBMCs and isolate T cells...

12 October 2020 3,583 6 View

May somebody help me to standardise the amount and time of the enzyme mixture to isolate Cytotrophoblast from placenta??

i tried with the published protocols, but i am not getting any seperation in the ficol gradient. It seems like i am not incubating the 7gm minced tissue for proper time in the digestion...

03 October 2020 8,354 4 View

Why are there so many platelets in PBMC of mice isolated by Ficoll, and What should I do to dislodge these paltelets?

Hi, We used Ficoll to isolate PBMC from mice whole blood cells, but there are many little nights,with no celler structures, in PBMC through a microscope. We guess these granules are paltelets, but...

29 September 2020 1,667 5 View

How safely isolate RNA from blood of COVID-19 positive patients?

We are going to islotate RNA from patients with severe COVID-19 symptoms. How to inactivate the virus potentially present in the blood? We do not have equipment dedicated to work with viruses in...

20 September 2020 7,417 3 View

How to separate monocytes by plastic adhesion?

07 July 2020 5,883 10 View

Can i use ficoll or percoll to isolate lymphocytes from murine spleen?

hi every one, i want to isolate antigen-specific CTL. my plan is as follows: 1. immunize mouse with peptide twice, at 2 weeks interval; 2. after 2weeks of second immunization, kill mouse and...

15 March 2020 4,311 3 View

What is the purpose for re-suspension after centrifugation during isolation of cells from whole blood?

I have been conducting an experiment which requires to isolate B lymphocytes from human whole blood. Several pieces of literature provide the procedure which includes re-suspension of lymphocytes...

01 February 2020 2,969 2 View

Can I any one sauggest me where can I purchase Urograffin/Renograffin in India?

I want to do density gradient centrifugation. I would like to purchase renografin/urograffin. None of the medical stores have it nor online it is available. I cant use sucrose or ficol. Could...

09 January 2020 9,315 3 View

Does anybody have an optimized protocol for isolating RNA from human plasma samples with Trizol-LS?

31 December 2019 5,534 5 View