Hind Sebbah

7 Questions 6 Answers 0 Followers

Questions related from Hind Sebbah

Which contamination in these flasks?

I'm culturing THP-1 cells in a medium composed of 90% RPMI, 10% FBS, and 1% antibiotics (penicillin and streptomycin). When I checked my cells, I noticed some contamination (see picture), but I...

11 October 2024 6,856 0 View

Wich tubes of PAXgene Blood RNA Tube?

Does anyone know which tubes the PAXgene Blood RNA Tube corresponds to? Are they EDTA tubes or Heparin tubes? Best regards, Hind Sebbah

16 January 2024 3,327 1 View

Free Graphpad for Mac?

Dear researchers, I would like to ask you if anyone have a cracked version of Graphpad for Mac. Because I'm absolutely needing it, I don't have the free version anymore. Best regards Hind Sebbah

25 December 2023 5,754 0 View

I am currently culturing THP-1 cells in a T75 flask. Can I have 32 millions cells in 20ml?

Dear researchers, I am currently culturing THP-1 cells in a T75 flask. I would like to inquire whether it is possible to achieve a confluence of 100% with 32 million THP-1 cells in 20 ml of...

04 December 2023 8,098 2 View

Isolation of PBMC with Lymphoprep with RPMI from blood from citrate tubes?

Dear researchers, I have isolated PBMCs from whole blood using EDTA tubes with Lymphoprep. I followed the protocol, but I have come across some forums suggesting the dilution of blood with RPMI,...

26 November 2023 10,004 4 View

Which density of THP-1 in culture in flask?

Dear researchers, I don't have any experience with the culture of THP-1 cells. I have found that the optimal confluence to begin a culture is 1x10^6 THP-1 cells/ml, and approximately after 1...

19 November 2023 4,746 2 View

How many time do I culture primary monocytes after isolation with microbeads CD14+ for stimulation with LPS?

I read in some articles that after isolation of primary monocytes I must culture them for a couple of hours or overnight and after that I can stimulate them with LPS. Why? For adherence? The...

12 November 2023 9,709 2 View