Hello ResearchGate Community,

I am optimizing a panel of probes/primers for the detection of 14 HPV types.

What criteria would you suggest to choose the reference sequence from all reported sequences on the NCBI?

Would you choose the most recent version, the ones with the highest lengths, the sequence with the least unspecified nucleotides, those most frequently cited by papers, etc.?

It means a whole world to receive your suggestions/ ideas in this regard.

Eagerly look forward to hearing back from you.

Best,

Negar

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