Hello.
A protein dimer forms how many band in native PAGE, 1 or 2?
Dear everybody Hello. What is the definition of hydrodynamic radius of proteins? Thanks
09 October 2016 6,115 0 View
Hello all Dose anybody know the MW of three isoforms of carbonic anhydrase in native state? And can it be used as marker in non-denaturing PAGE to estimate the native MW of an unknown protein by...
08 September 2016 9,721 0 View
Hello Dose electric current itself dissociate protein subunits? If we observe 2 bands in native PAGE, we dealt with 2 proteins or with a dimer?
07 August 2016 5,790 3 View
Hello. I have run heat-denatured RNA in 1% agarose gel in TBE×0.5 running buffer that both gel and running buffer contained 1 M Urea. After illumination by UV, gel background appeared some white...
07 August 2016 6,948 3 View
What happens if we run native protein in SDS-PAGE?
06 July 2016 9,439 0 View
I have isolated a plant protein fraction forming four MW closely-related bands in SDS-PAGE, ranging 30-35 KDa. In the absence of 2-ME, the fraction resolved as 2 bands with MWs of about 28 and 30...
01 January 1970 7,448 3 View
I'm currently working on calculating the collision cross section (CCS) for various ions, and I'm facing challenges when dealing with sodiated and multiply charged ions. Most of the resources I’ve...
08 August 2024 8,329 0 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
Currently, when I run SDS-PAGE, I don't see any bands at all, even though I used the same material just a day ago and it worked fine.... In our lab, we dilute the 10X running buffer to 1X and...
06 August 2024 5,373 2 View
Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...
06 August 2024 1,989 3 View
I ran a SDS-page of a bacterial lysate and I want to quantify protein concentration in a specific band. I was thinking of using a standards ladder or make some standards are different...
05 August 2024 9,805 3 View
Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC
01 August 2024 967 3 View
Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...
31 July 2024 9,892 4 View
I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...
30 July 2024 6,420 1 View
I am using CuBr/THPTA for a click reaction in total cell lysates. I am facing issues with my protein sample in non-reducing SDS-PAGE where it's not migrating properly and most of it remains at the...
29 July 2024 950 4 View
Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....
29 July 2024 5,007 0 View