Number of dimer?

Hello. A protein dimer forms how many band in native PAGE, 1 or 2?

09 October 2016 4,445 0 View

Native MW of carbonic anhydrase?

Hello all Dose anybody know the MW of three isoforms of carbonic anhydrase in native state? And can it be used as marker in non-denaturing PAGE to estimate the native MW of an unknown protein by...

08 September 2016 9,665 0 View

Protein subunit in native PAGE?

Hello Dose electric current itself dissociate protein subunits? If we observe 2 bands in native PAGE, we dealt with 2 proteins or with a dimer?

07 August 2016 5,727 3 View

Electrophoresis of RNA in denaturing conditions?

07 August 2016 6,896 3 View

Native or SDS-PAGE?

What happens if we run native protein in SDS-PAGE?

06 July 2016 9,378 0 View

Protein subunits in SDS-PAGE?

I have isolated a plant protein fraction forming four MW closely-related bands in SDS-PAGE, ranging 30-35 KDa. In the absence of 2-ME, the fraction resolved as 2 bands with MWs of about 28 and 30...

01 January 1970 7,391 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

How to Eliminate cosine error and abbe error in LASER measurement system(for example Rhenishaw XL80)?

I like to do project in laser measurement system. My objective is to eliminate or reduce the cosine error, abbe error in Laser measurement system. I have Rhenishaw XL80 system. Please tell the...

28 February 2021 4,890 3 View

How can we limit artifacts caused by rotation in fiber photometry?

We are trying to run fiber photometry experiments for long time scales, but we get these sinusoidal artifacts that come up. We think they may be caused by twisting of the fiber optic subject...

25 February 2021 568 1 View

How to measure angle between laser travel path and machine axis in Laser Measurement system?

25 February 2021 1,987 3 View

What's the concentration recommend for recombinant protein use in western blotting?

I'm currently working having problem with the concentration for recombinant protein use in western blot, the one been used is Recombinant Human CD19 Fc Chimera Protein-rndsystem which does not...

24 February 2021 8,514 3 View

How can I simulate the optical absorption spectrum of a biomaterial (like DNA or protein) in COMSOL?

I need to simulate the optical properties of a biomaterial, namely DNA, in COMSOL. Basically I need to simulate the IR absorption spectrum of a cylinder filled with a 'concentration' of DNA. I am...

21 February 2021 3,043 3 View

Will SARS-CoV-2 Spike be broken into S1 and S2 by denaturing conditions?

Hello, I am performing a western blot of my SARS-CoV-2 Spike-pseudotyped lentiviral particles. I denature my virus by adding concentrated virus to 2X Laemmli buffer at 1:1, then boiling for...

17 February 2021 4,844 4 View

Kinetics of Enzyme production in Recombinant E Coli?

Hello, I am trying to find kinetic information (such as rate of enzyme production etc.) on the expression of taq polymerase in E-Coli after inserting a recombinant plasmid. Any help would be...

12 February 2021 1,386 3 View

Is ANSYS Fluent good for simulation of a three phase reactor(stusy of its hydrodynamics or mass transfer...)?

Hello everybody, I would like to simulate a three phase reactor and study its hydrodynamics. I would like to know which type of software is better? I have a good command of ANSYS Fluent and I am...

10 February 2021 4,303 3 View

How to measure protein prescence in a tile scan in ImageJ?

Hello all, I have attached an image in which I would like to know the IntDen and/or the amount of protein particles. The wells are custom made, thus looking slightly warped sometimes. So far my...

10 February 2021 3,478 3 View