Hydrodynamic radius of proteins?

Rasoul Rasoulpour @Rasoul_Rasoulpour2

10 October 2016 0 6K Report

Dear everybody

Hello. What is the definition of hydrodynamic radius of proteins?

Thanks

Badges
Science topic

More Rasoul Rasoulpour's questions See All

Number of dimer?

Hello. A protein dimer forms how many band in native PAGE, 1 or 2?

09 October 2016 4,502 0 View

Native MW of carbonic anhydrase?

Hello all Dose anybody know the MW of three isoforms of carbonic anhydrase in native state? And can it be used as marker in non-denaturing PAGE to estimate the native MW of an unknown protein by...

08 September 2016 9,721 0 View

Protein subunit in native PAGE?

Hello Dose electric current itself dissociate protein subunits? If we observe 2 bands in native PAGE, we dealt with 2 proteins or with a dimer?

07 August 2016 5,790 3 View

Electrophoresis of RNA in denaturing conditions?

Hello. I have run heat-denatured RNA in 1% agarose gel in TBE×0.5 running buffer that both gel and running buffer contained 1 M Urea. After illumination by UV, gel background appeared some white...

07 August 2016 6,948 3 View

Native or SDS-PAGE?

What happens if we run native protein in SDS-PAGE?

06 July 2016 9,439 0 View

Protein subunits in SDS-PAGE?

I have isolated a plant protein fraction forming four MW closely-related bands in SDS-PAGE, ranging 30-35 KDa. In the absence of 2-ME, the fraction resolved as 2 bands with MWs of about 28 and 30...

01 January 1970 7,448 3 View

Problems about acousto-optic coupling in Comsol, how to check whether the moving mesh is correct?

I’m trying to reproduce the acousto-optic coupling simulation in the paper Frequency–angular resolving LiDAR using chip-scale acousto-optic beam steering. LNOI(lithium niobate on insulator) is...

13 August 2024 9,795 0 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

What is the best blank for nanodrop if I want to read a recombinant protein concentration?

Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC

01 August 2024 967 3 View

Can a Conventional 50W visible LED Lamp Emit UV Light?

I'm researching light sources and their UV emissions. I'm curious if a 50W, 7500K conventional LED lamp emits any UV light. I found that a 200W tungsten lamp can emit up to 1.5 mW/cm² at 350 nm....

31 July 2024 3,843 7 View

Problems with ion exchange chromatography?

I'm having problems with ion exchange chromatography. After applying my sample (a fluorescent protein), a preferential path inevitably forms. The matrix I use is DEAE-Sephacel. Could someone...

27 July 2024 6,605 0 View

How do regenerate Amberlite XAD 4 resin for further cycles of use for removing 0.5% Sarkosyl from recombinant protein solutions?

I use 0.5% sarkosyl to solubilise recombinant proteins contained in Inclusion bodies of E. coli. sarkosyl very effeciently solubilizes proteins from Inclusion bodies and I am trying to use...

25 July 2024 5,001 2 View

How do nutrient transporters and aquaporins interact under abiotic stress in plants?

"The Correlation between Nutrition and Transport Mechanism under Abiotic Stress in Plants: A Comprehensive Review" by Muhammad Saleem, Jianhua Zhang, Muhammad Qasim, Rashid Iqbal, and Li Song,...

23 July 2024 9,109 0 View

How Do Length of Pylon and Rotor Disk Radius Impact Angular Frequency and Torsional Stiffness in Whirl Flutter Analysis for a 2 DOF Propeller System?

Actually, I'm having trouble implementing the plot of the flutter region for the whirl prop system with 2 DOF (Influences of structural damping and propeller—pivot point distance on whirl flutter...

21 July 2024 5,047 2 View

Consequences of excess bases in the MCS after digestion?

Good morning, I am trying to produce a recombinant protein. Unfortunately I one of the two restriction enzymes that I use do not cut at the end of the MCS. After the digestion 15 nucleotides of...

17 July 2024 2,787 4 View

The hydrodynamic evolution on the coastline of west Point, Monrovia, Liberia?

the hydrodynamic For the past 20 years, from 2003 to 2023, the present year, 2024 and future year 2040.

10 July 2024 6,044 0 View