Hello all
Dose anybody know the MW of three isoforms of carbonic anhydrase in native state? And can it be used as marker in non-denaturing PAGE to estimate the native MW of an unknown protein by linear regression?
Thanks
Dear everybody Hello. What is the definition of hydrodynamic radius of proteins? Thanks
09 October 2016 6,115 0 View
Hello. A protein dimer forms how many band in native PAGE, 1 or 2?
09 October 2016 4,502 0 View
Hello Dose electric current itself dissociate protein subunits? If we observe 2 bands in native PAGE, we dealt with 2 proteins or with a dimer?
07 August 2016 5,790 3 View
Hello. I have run heat-denatured RNA in 1% agarose gel in TBE×0.5 running buffer that both gel and running buffer contained 1 M Urea. After illumination by UV, gel background appeared some white...
07 August 2016 6,948 3 View
What happens if we run native protein in SDS-PAGE?
06 July 2016 9,439 0 View
I have isolated a plant protein fraction forming four MW closely-related bands in SDS-PAGE, ranging 30-35 KDa. In the absence of 2-ME, the fraction resolved as 2 bands with MWs of about 28 and 30...
01 January 1970 7,448 3 View
I did PR spin coating on trench structure. I used AZ P4620 PR and the thickness or PR is around 11um. The substrate is Si. And my trench structure depth is 141um(negative way). Even though I...
08 August 2024 2,298 0 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
I am trying to analyse data from a survey examining what variables affect teachers perceived barriers to incorporating technology into their classroom. I have 5 predictor variables however my DV...
06 August 2024 1,752 3 View
I am using unit level data (IHDS round 2) & Stata 17
06 August 2024 5,725 2 View
Hi everyone, If you have written or come across any papers where Generalised Linear Mixed Models are used to examine intervention (e.g., in mental health) efficacy, could you please share the...
04 August 2024 4,130 4 View
- The Existence/Uniqueness of Solutions to Higher Order Linear Differential Equations - Higher Order Homogenous Differential Equations - Wronskian Determinants of $n$ Functions - Wronskian...
03 August 2024 2,366 0 View
Reference dose and Maximum acceptable concentrations HMs
03 August 2024 8,230 4 View
I used humic acid at 0.044 g/kg soil in my pot experiment. But finally, I have to recommend kg/ha. Each pot's soil weight was 11 kg. What is the solution?
02 August 2024 7,186 6 View
Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC
01 August 2024 967 3 View
When we conduct linear regression, there are several assumptions. The assumption of normality is whether the residual errors are normally distributed, not whether a predictor is normal?
31 July 2024 6,164 3 View