Hello. I have run heat-denatured RNA in 1% agarose gel in TBE×0.5 running buffer that both gel and running buffer contained 1 M Urea. After illumination by UV, gel background appeared some white and RNA bands resolved weak and a little diffuse. This problem was not existed in non-denaturing conditions using the same concentration of RNA (3  µ g) per lane. So, how can I improve the problem?

Thanks

Similar questions and discussions