Hi all,
I am curious about any notable mutations in the Capan-2 pancreatic adenocarcinoma cell line. I know Capan-2 has a KRAS mutation, but are there any other notable mutations, such as of tumor suppressor genes, that I should be aware of?
Thanks.
It is notable that Capan-2 has a normal wild-type p53, while Panc-1 and MIA PaCa-2 have mutant p53.
Anticancer Res. 2002 Mar-Apr;22(2A):825-30.
Influence of p53 status on radiation and 5-flourouracil synergy in pancreatic cancer cells.
Hello Frank
Hello Go
Go, you have to be very careful with your information and when you reply on a forum. Capan-2 cell line is not wt for TP53 gene. It has a mutation at codon 125.
chr17:g.7579312G>T ( c.375G>T , p.T125= )
This synonymous mutation close to the intron junction is very well known to be deleterious as it changes TP53 splicing.
Several publications have described this mutation in Capan-2 cell line and it is also described in the cosmic database.
The best source for TP53 (and other genes) info in cell line is the Cosmic database:
http://cancer.sanger.ac.uk/cell_lines
NEVER EVER RELY ON A SINGLE PUBLICATION FOR THE GENETIC STATUS OF A CELL LINE.
Leroy, B., Girard, L., Hollestelle, A., Minna, J. D., Gazdar, A. F., and Soussi, T. (2014). Analysis of TP53 mutation status in human cancer cell lines: a reassessment. Hum Mutat 35, 756-765.
Berglind, H., Pawitan, Y., Kato, S., Ishioka, C., and Soussi, T. (2008). Analysis of p53 mutation status in human cancer cell lines: a paradigm for cell line cross-contamination. Cancer Biol Ther 7, 699-708.
As shown in the Cellosaurus entry:
https://web.expasy.org/cellosaurus/CVCL_0026
There are mutations in both KRAS and TP53.
As I am in the process of adding notable mutations in the Cellosaurus, will check if there is something else to add to Capan-2
Potentially (need to be checked) there is also a CDKN2A mutation:
http://cancer.sanger.ac.uk/cosmic/sample/overview?id=934554#variants
http://cancer.sanger.ac.uk/cosmic/sample/overview?id=932517#variants
Best
Amos
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