What is better for quantifying protein levels, ELISA or flow cytometry?

What are the advantages/disadvantages for each in quantifying intracellular protein levels?

05 June 2018 9,883 2 View

What assays can be used to detect cell cycle arrest in culture?

Preferably assays involving flow cytometry. Thanks.

05 June 2018 9,677 3 View

Best protocol to use for protein level detection?

There are many methods being used in the industry to measure protein levels: from Western Blots, to ELISA, to RT-PCR, to immunoblot, to flow cytometry. Could I have a clarification on the contexts...

04 May 2018 962 3 View

Cell seeding density for Senescence-Associated Beta Galactosidase Assay?

Hi all, Does anyone know if there has to be a specified seeding density for the senescence-associated beta galactosidase assay performed in a 96-well plate? Or does any seeding density generally...

03 April 2018 9,638 1 View

Can p53 Activation induce more replicative senescence than apoptosis?

Hello all, I recently treated telomerase-inhibited A549 cells with 5 uM RITA, a p53 activator drug, and measured cell viability using the MTT assay. While wild type A549 experienced an expected...

02 March 2018 3,019 4 View

Proper PCR DNA Polymerase to Use For DNA seqeuncing?

Hi all, When I am conducting PCR in order to amplify a certain DNA segment for subsequent Sanger sequencing, would using the conventional DreamTaq Green Mix from Thermo Fisher work fine? Or...

01 February 2018 9,140 3 View

Assays to perform for measuring replicative senescence?

Hello all, I am performing telomerase inhibition experiments that will result in replicative senescence in cancer cell culture. Can anyone recommend assays to use to measure replicative...

01 February 2018 4,206 2 View

Why must cancer xenografts occur in immunodeficient mice?

Hello, I'm curious as to why cancer xenografts must occur in immunodeficient mice. Ordinarily would the mouse immune system eliminate the cancer cells? And why would this not occur in regular...

31 December 2017 1,456 5 View

Why did A549 Cell Viability Increase upon treatment with p53 activator drug RITA?

I treated wild type A549 cells with 10 nM RITA, a p53 activator drug, and the cell viability increased 18.7% in a statistically significant manner (p

31 December 2017 1,160 2 View

Notable mutations in Capan-2 pancreatic adenocarcinoma cell line?

Hi all, I am curious about any notable mutations in the Capan-2 pancreatic adenocarcinoma cell line. I know Capan-2 has a KRAS mutation, but are there any other notable mutations, such as of tumor...

31 December 2017 1,571 3 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View