Cell seeding density for Senescence-Associated Beta Galactosidase Assay?

04 April 2018 1 10K Report

Hi all,

Does anyone know if there has to be a specified seeding density for the senescence-associated beta galactosidase assay performed in a 96-well plate? Or does any seeding density generally work for the assay? Thanks!

Katharina Iwanov

Hej Frank,

depending on the protocol or kit you are using:

if there is no information given there, I would go for whatever is "healthy" for your cell line; for most cell lines this would be something between 60 and 80% confluence - you will most definitely find detailed info on that for your specific cell line online!

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