What is better for quantifying protein levels, ELISA or flow cytometry?

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Mokhtar Nosrati

Hi, both methods can be used for protein quantification but ELISA is more reliable. This article may be helpful.

Article Comparison of cell-ELISA, flow cytometry and Western blottin...

Gunay Aliyeva

Flow cytometry is rather for cell based assays. Also good for the detection of intracellular or membrane proteins. If you need to quantify the level of soluble protein, ELISA is cheaper, easy to learn and more practical.