What is better for quantifying protein levels, ELISA or flow cytometry?

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Preferably assays involving flow cytometry. Thanks.

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Can p53 Activation induce more replicative senescence than apoptosis?

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Proper PCR DNA Polymerase to Use For DNA seqeuncing?

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Buffer to disrupt platelet alpha granules for PF4 quantification?

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Sample dilution for quantitative analysis using ELISA?

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What is the characteristic length to determine the Reynolds number for the scattered turbulent fluid flow?

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Issues with Permeabilization during Flow Cytometry?

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Are protein samples in SDS compatible with ELISA?

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Anyone know of a good progesterone antibody?

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Benefits of the 24 welled plate?

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Why do the air flow results vary totally when adding scalar in CFD ?

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Unknown debris sheets observed in cell culture flasks?

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Mokhtar Nosrati

Hi, both methods can be used for protein quantification but ELISA is more reliable. This article may be helpful.

Article Comparison of cell-ELISA, flow cytometry and Western blottin...

Gunay Aliyeva

Flow cytometry is rather for cell based assays. Also good for the detection of intracellular or membrane proteins. If you need to quantify the level of soluble protein, ELISA is cheaper, easy to learn and more practical.