16 Questions 5 Answers 0 Followers
Questions related from Frank Liu
What are the advantages/disadvantages for each in quantifying intracellular protein levels?
06 June 2018 9,932 2 View
Preferably assays involving flow cytometry. Thanks.
06 June 2018 9,709 3 View
There are many methods being used in the industry to measure protein levels: from Western Blots, to ELISA, to RT-PCR, to immunoblot, to flow cytometry. Could I have a clarification on the contexts...
05 May 2018 995 3 View
For anyone who has worked with this cell line before, could you please confirm if Capan-2 is a format of pancreatic ductal adenocarcinoma? Thanks.
04 April 2018 962 2 View
Hi all, Does anyone know if there has to be a specified seeding density for the senescence-associated beta galactosidase assay performed in a 96-well plate? Or does any seeding density generally...
04 April 2018 9,674 1 View
Hello all, I recently treated telomerase-inhibited A549 cells with 5 uM RITA, a p53 activator drug, and measured cell viability using the MTT assay. While wild type A549 experienced an expected...
03 March 2018 3,049 4 View
Hi all, When I am conducting PCR in order to amplify a certain DNA segment for subsequent Sanger sequencing, would using the conventional DreamTaq Green Mix from Thermo Fisher work fine? Or...
02 February 2018 9,174 3 View
Hello all, I am performing telomerase inhibition experiments that will result in replicative senescence in cancer cell culture. Can anyone recommend assays to use to measure replicative...
02 February 2018 4,236 2 View
Hi all, I am curious about any notable mutations in the Capan-2 pancreatic adenocarcinoma cell line. I know Capan-2 has a KRAS mutation, but are there any other notable mutations, such as of tumor...
01 January 2018 1,604 3 View
Hello, I'm curious as to why cancer xenografts must occur in immunodeficient mice. Ordinarily would the mouse immune system eliminate the cancer cells? And why would this not occur in regular...
01 January 2018 1,486 5 View
I treated wild type A549 cells with 10 nM RITA, a p53 activator drug, and the cell viability increased 18.7% in a statistically significant manner (p
01 January 2018 1,190 2 View
Can someone provide to me a brief summary on the functions of TRF1 within the mammalian shelterin complex? Also, if known, what are the effects of TRF1 inhibition or increased expression? While...
12 December 2017 9,405 0 View
I'm wondering if anyone has ever observed ALT expression in adenocarcinoma cell lines. A prior published paper studying many cancer types never showed any ALT epxression in adenocarcinomas in...
12 December 2017 3,808 1 View
Hi everyone, does anyone know of any lipase enzymes found in nature that have the ability to degrade mycolic acid? Do normal lipases such as human pancreatic lipase (HPL) have the ability to...
08 August 2017 9,987 1 View
I have a 64-bit Windows machine that I tried downloading Flowjo onto, but after installing when I double click to open the program nothing happens. I have tried installing both the 32- and 64- bit...
08 August 2017 1,751 3 View
Trying to save cost here...can I grow tissue culture in a non-CO2 incubator as long as the media is supplemented with HEPES as a buffer instead? Thanks.
07 July 2017 5,704 3 View
