hey,
I would like to buy My-La cells,
and I'm not sure which one should I buy.
I saw in the literature that there is malignant and non-malignant My-La cells,
I'm looking for the malignant strain.
Does anyone here worked with those cells and can advise me what should I do?
Thanks,
Hadas
Colleagues of mine have done work in this area. The novel "discovery" of the non-malignant My La cell lines is well described in this article (free access via pubmed to article and confirmation of the two cell lines that can be induced by SE to stimulated the malignant cell line. The link to the various cell lines related and their characteristics is also freely accessible.
The malignant T-cell line MyLa 2039 and the MyLa 2059 subline were established from a plaque biopsy specimen of a patient with MF. The MyLa 2059 cell line has been kept in long-term culture and shows some karyotypic changes.
The malignant T-cell line MyLa 1929 was established from an independent biopsy (3 month later) and resembles closely (karyotypically and otherwise) the MyLa 2039 cell line and the original tumor.
Reliable Distributors of various malignant cell lines are ordered for research purposes only. Their website will explain culture conditions and details phenotypic surface marker, genotypic and karotypic and other characteristics that may be important to you. If you wish to commercialize a product by using this cell line, they provide the link to the depositor of the cell line whose permission you must obtain.
e.g. "Two different continuous cell lines were established from a plaque biopsy of an 82-year old male with mycosis fungoides stage II by inclusion of IL-2 and IL-4 in the culture medium: My-La, CD4+, (referred to in the literature as My-La, marker chromosome) and My-La, CD8+ (referred to in the literature as My-La 46,XY-18+i(18q) (Sigma Catalogue number. 95051033). My-La CD4+ is strictly dependent on exogenous IL-2 and IL-4 in the culture medium whereas My-La CD8+ on IL-2 only. Both show characteristic chromosome markers and are double homozygous for HLA-A1.B8. My-La CD8+ expresses β-13 of the T cell receptor and phenotypically weak staining was observed for CD2, CD3, CD8, CD25, CD71 and TCR-1 α / ß. My-La CD4+ expresses β-18 of the T cell receptor plus staining for CD2, CD3, CD4,CD25, CD71, HLA-DR and TCR-1 α/ ß. There has been no evidence of HTLV-I infection in the cell lines and serum from the patient did not demonstrate antibodies against HTLV-I and HTLV-II. Both cell lines show a low viability after resuscitation from frozen and a long lag time phase until exponential growth is reached."
University of MIchigan has a resource for nomenclature and origins of various cell lines that are developed. The Ontobee link is below for the My La 2059, and the 2039 malignant cell line was established from that one line.
Hope this is useful for you!
Best,
Alan
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1852254/
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1852254/table/T1/
http://www.ontobee.org/ontology/BTO?iri=http://purl.obolibrary.org/obo/BTO_0004843
Hello,
I hate to dig up an old post, but I was wondering if anyone could explain the difference between the MyLa cells offered by Sigma/ECACC (MyLa CD4+ and MyLa CD8+) and the MyLa 2059 subline. We are looking to acquire MyLa 2059 cells but are stumped by this question. Thank you!
- Badges
- Science topic
- Similar topics
- Biological Science
- Molecular Cell Biology
- Culture Cells
- Cell Line