How come the isotype signal is lower than the basal signal when analyzing flow cytometry?

Hi everyone I have performed several flow cytometry experiments and I keep getting a basal FITC signal (of unstained cells) higher than FITC isotype control signal (of cells stained with the...

26 July 2020 8,439 3 View

How do I quantify capsular polysaccharide in bacteria?

Hi. Is there a reliable protocol / method / kit for quantification of capsular polysaccharide in Acinetobacter baumannii or any other bacteria for that matter? preferably without the use of...

25 September 2019 7,303 3 View

No title

25 June 2019 498 2 View

Immunofluorescence - can I use a conjugated antibody and a primary not-conjugated different antibody - both from the same host species?

Has anyone ever tried to stain 2 different proteins in a single sample, while they both originate from he same species (1 of them conjugated though)? I have 1 primary antibody (host: mouse)...

06 May 2019 6,551 6 View

My-La cell line

hey, I would like to buy My-La cells, and I'm not sure which one should I buy. I saw in the literature that there is malignant and non-malignant My-La cells, I'm looking for the malignant...

17 February 2016 4,972 3 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

How to avoid ulcers in s.c. injected tumors ?

Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...

01 March 2021 9,938 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View