Does anyone have ever found a spontaneous expression of mast cells in Sash mice?

Hi all, I am working with Sash mice and in one of my experiments I saw two Sash mice that was double stained to cKIT and FCeRI. I was wondering if during time the cKit mutation can be lost? Does...

20 September 2020 6,876 2 View

How come the isotype signal is lower than the basal signal when analyzing flow cytometry?

Hi everyone I have performed several flow cytometry experiments and I keep getting a basal FITC signal (of unstained cells) higher than FITC isotype control signal (of cells stained with the...

26 July 2020 8,439 3 View

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25 June 2019 498 2 View

Can we use skin keratinocyte (HaCaT cell line) to assess cytotoxicity of extract using SRB method?

SRB assay has been used with cancer cell lines to assess anti-cancer activity of extract, but is it correct to apply this SRB assay on skin keratinocyte to assess cytotoxicity?

07 May 2019 665 1 View

Immunofluorescence - can I use a conjugated antibody and a primary not-conjugated different antibody - both from the same host species?

Has anyone ever tried to stain 2 different proteins in a single sample, while they both originate from he same species (1 of them conjugated though)? I have 1 primary antibody (host: mouse)...

06 May 2019 6,551 6 View

My-La cell line

hey, I would like to buy My-La cells, and I'm not sure which one should I buy. I saw in the literature that there is malignant and non-malignant My-La cells, I'm looking for the malignant...

17 February 2016 4,972 3 View

How about the performance of TAMRA quencher?

Has anyone tried using TAMRA quencher in the Taqman probe? I wonder if the fluorescence property of the TAMRA dye itself affects the result?

02 April 2014 9,054 2 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

How to compare different concentrations of nanoparticles for characterization from ICP-MS?

I took measurements of different concentrations of 15 nm and 5 nm Au nano particle solutions. I'm trying to determine which concentration I measured gives the best reading overall. I also...

02 March 2021 7,626 1 View

How to accurately quantify chitin?

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...

01 March 2021 1,843 2 View

What is the exact mechanism of the antimicrobial effect of local anesthetics?

The antimicrobial effect of local anesthetics has been known for more than 50 years, but it still seems to have an unclear mechanism of action. which bacteria? which concentration? etc etc. ....

27 February 2021 4,450 2 View

Uncertianiny in using dry or wet basis in mixing a sediment with fuel oil?

Hello I'm currently working on a topic related to studying the anaerobic biodegradation of TPH in marine sediments. In this work, sediment should be mixed with fuel oil to a concentration of 20 g...

24 February 2021 9,839 3 View

How can Gamma Ray API units be converted to CPS (counts per second)?

Oil and gas companies run gamma ray tool and take readings in API units while Gamma ray is also noted in cps, so how these units can be converted into each other?

24 February 2021 7,545 2 View

Advice on growing lactobacillus species in the lab?

Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...

24 February 2021 3,157 6 View

Best plasmid could be used as a standard curve for AAV titration?

I have been trying to have a good AAV titration for my production and I have tried different plasmids to set up as a standard curve but each one behaves differently. i am trying to use ITR for...

23 February 2021 5,167 3 View

Best solvent for L-DOPA extraction and HPLC analysis?

I'm working on Mucuna pruriens and want to quantify L-DOPA. Could someone please suggest the cheap and best solvent for the extraction and the solvent system that has to be used for its...

20 February 2021 6,058 2 View

What are the best cations for preparing competent cells, Ca2+ or Mg2+?

I have been reading various articles and most state that treatment with CaCl2 produces higher efficiency of transformation than MgCl2. Can anyone explain why Ca2+ is better than Mg2+ at...

16 February 2021 8,519 5 View