How do I quantify capsular polysaccharide in bacteria?

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Can cy5 be fixed using formaldehyde ?

Hello I am planning to deliver a cy5-labeled drug to mice, and then check its distribution in different organs. In case I wish to preserve the tissues for later research, will fixation (using 4%...

12 November 2023 4,958 1 View

Can BSA alone increase inflammation in macrophages?

Hello, I am studying the effect of different fatty acids on LPS induced macrophages (J774A1 and THP-1). I use saponified fatty acids in complex with BSA, but observed that while i would like to...

12 February 2022 5,721 0 View

Does anyone have ever found a spontaneous expression of mast cells in Sash mice?

Hi all, I am working with Sash mice and in one of my experiments I saw two Sash mice that was double stained to cKIT and FCeRI. I was wondering if during time the cKit mutation can be lost? Does...

20 September 2020 7,005 2 View

How come the isotype signal is lower than the basal signal when analyzing flow cytometry?

Hi everyone I have performed several flow cytometry experiments and I keep getting a basal FITC signal (of unstained cells) higher than FITC isotype control signal (of cells stained with the...

26 July 2020 8,567 3 View

FIXATION TIME before tissue slicing for paraffin infiltration - how much time?

Hi I need to make paraffin blocks to my murine hearts (extracted from 3 month old mice). Following extraction the hearts were held in 4% formaldehyde and 2 days later transferred to 70% EtoH. I...

19 April 2020 7,509 0 View

Endothelial cells present in fibroblasts primary culture from mouse?

Hi, I isolated cardiac fibroblasts from a mouse and cultured them in P.1. After mRNA extraction I saw high expression levels of CD146 gene, so I wonder if at that point it's possible that ECs...

07 July 2019 5,249 3 View

Can cells kept in -80°C be used for western blotting?

Hi, I reached to the point I had spare cell-cultures, so I kept some of the cell plates in -80°C (no medium of-course, after cold PBS washing - I removed PBS to be left only with cells). Now I...

25 June 2019 660 2 View

Can we use skin keratinocyte (HaCaT cell line) to assess cytotoxicity of extract using SRB method?

SRB assay has been used with cancer cell lines to assess anti-cancer activity of extract, but is it correct to apply this SRB assay on skin keratinocyte to assess cytotoxicity?

07 May 2019 782 1 View

Immunofluorescence - can I use a conjugated antibody and a primary not-conjugated different antibody - both from the same host species?

Has anyone ever tried to stain 2 different proteins in a single sample, while they both originate from he same species (1 of them conjugated though)? I have 1 primary antibody (host: mouse)...

06 May 2019 6,666 6 View

Where can I find information about the Achaemenid naval?

For an academic lecture for one of my courses I'm studying in the university.

26 December 2018 3,255 0 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

How can I inoculate a lyophilized anaerobic bacteria into the culture medium without an anaerobic chamber?

There are anaerobic gas pak jars and environment providers in the laboratory that provide anaerobic environmental conditions for the growth of anaerobic bacteria. However, there is no anaerobic...

05 August 2024 4,945 3 View

How to analyze multiple phosphorilation sites?

Hello, I am currently analyzing some phosphoproteomics data, but I have peptides with multiple phosphorylation sites or phosphorylations together with carbamidomethylation or oxidation. How can I...

04 August 2024 8,432 3 View

Can the limit of quantification (LOQ) of an analytical method fall outside its linear dynamic range, or must it always be within it?

Can an analytical method's limit of quantification (LOQ) be outside its linear dynamic range, or is it always required to be within it? Please provide a thorough explanation supported by verified...

29 July 2024 7,198 9 View

How does the Retention time effect the Biogas Production?

List down the various factors responsible and how does this effect the overall biogas production when it is related to Hydraulic retention time of the digester based on the feedstock?

27 July 2024 4,800 4 View

Question about MHB (Mycorrhiza Helper Bacteria) isolation technique??

What are the most effective isolation techniques for obtaining MHB?? Is there are best approach techniques? I keep struggling in obtaining it.

26 July 2024 7,387 0 View

Prediction and quantification of water content ?

Hi all. Is it possible to correlate multiple parameters like fruit weight, color, length and diameter, its ratio with water content in coconut ?. Need some guidance on some non destructive methods...

23 July 2024 9,421 3 View

Pink bacterial colonies?

I am cloning an overexpression plasmid with my protein of interest tagged with mScarlet. After transforming my ligated product into DH5α bacteria and plating on LB agar, I noticed colonies with a...

22 July 2024 5,953 6 View

What does negative readings in spectrophotometer denote. Was testing TSS content of bacterial cultures?

I was performing estimation of Total Soluble Sugar content in bacterial cultures by Anthrone method. Out of 9 samples, only one sample showed positive reading while others had a negative reading...

22 July 2024 9,576 4 View

How can I decrease the error of Rietveld method for phase quantification?

Phase quantification in steel contain BCC and FCC

20 July 2024 8,150 7 View