After following the exact protocol of the NucleoSpin Plasmid purification kit, I measured the concentration to find the 260/280 ratio 2.15! Any idea what could have gone wrong and how can I fix this?
RNA will give higher 260/280 ratio. Using more or fresh RNase with longer time incubation may solve your problem!
I wouldn't consider a 260/280 ratio of 2.15 that alarming. A slightly basic elution buffer can increase the 260/280 ratio by as much as 0.3. If possible, ensures your blank buffer and your sample buffer are the same pH. Typically, protein or phenol contamination would result in a ratio that is much lower than 1.8. I would be concerned if you were getting less than 1.5. Here's some additional info:
http://www.bio.davidson.edu/projects/gcat/protocols/NanoDrop_tip.pdf
http://biomedicalgenomics.org/RNA_quality_control.html
Here is a blog with details on plasmid prep protocol optimization http://blog.addgene.org/optimizing-plasmids-101-plasmid-yields
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