Debasish Malik

2 Questions 3 Answers 0 Followers

Questions related from Debasish Malik

Does differently efficient primer pair affect the absolute quantification in Real time pcr ?

I am trying to measure the quantity of 4 variants of a gene. These variant has some common sequence, I designed a primer at common sequence and the other in uncommon sequence, This way I will...

03 March 2018 5,759 3 View

How do isolate RNA from single myofiber?

I am trying to isolate RNA from single myofiber with trizol but it does not worked. How do I isolate RNA from single tiny myofibers. If anyone has any protocol or suggestions please share.

02 February 2017 8,235 3 View