What is the correct equation to use when weighing the different chemical shifts of 1H and 15N nuclei in SOFAST HMQC spectra?

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Engi Hassaan

Thanks a lot for your answer. I noticed some papers multiply the δ1H squared and δ15N squared by 1/2 and then find the root square of their sum. Why multiply with 1/2?

Giovanni Bellomo

This publication may help you:

https://www.sheffield.ac.uk/polopoly_fs/1.393312!/file/shift_mapping_2013.pdf

I usually do: δavg=sqrt( 0.5*δ1H^2+0.5*(δ15N/5)^2)

What is a self intensity test in STD NMR?

Is there a program I can use to align lattice constants of a protein crystal?

After my TEV cleavage site I have a remaining 'am' sequence. Where does this come from?

No title

Has anyone tried to add IPTG to auto inducing media like ZYM? Did you notice an increase in protien expression or is it pointless?

Effect of 1000 folds antibiotic addition to e. coli culture media?

My DNA plasmid purification yielded a 260/280 ratio of 2.15. Why?

Can I re-induce protein expression after storage at 4 degrees Celsius?

Timeout error!! Processor in the loop (PIL) using STM32F4 Matlab 2018a?

No title

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Has anyone used deep learning for 2D/3D REGISTRATION ?

absorption= 1-mag(S(FP1:1,FP1:1)*S(FP1:1,FP1:1))-mag(S(FP2:1,FP1:1)*S(FP2:1,FP1:1)) is it correct for absorption ?

absorption= 1-mag(S(FP1:1,FP1:1)S(FP1:1,FP1:1))-mag(S(FP2:1,FP1:1)S(FP2:1,FP1:1)) is it correct for absorption ?