Is there a program I can use to align lattice constants of a protein crystal?

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What is a self intensity test in STD NMR?

Some papers report performing a "STD self intensity" test to check the aggregation of compounds in STD NMR. Can anyone please elaborate how such a test is performed?

07 August 2019 6,949 2 View

What is the correct equation to use when weighing the different chemical shifts of 1H and 15N nuclei in SOFAST HMQC spectra?

I read in the attached paper the equation for HSQC spectra (equation 8). What about HMQC? I cannot find a reference online. Thanks!

04 May 2019 9,901 2 View

After my TEV cleavage site I have a remaining 'am' sequence. Where does this come from?

I am purifying a protein with a his tag. The TEV cleavage site I read is composed of ENLYFQG, and this G remains after cleavage. What I found to also remain is an 'am'. It is before the first...

09 October 2017 1,565 2 View

I stored my lysate at 4°C, can I still use it or toss it out?

I have lysed my e. coli cells (after adding protease inhibitors to the lysis buffer) and freezed the lysate. I then unfroze with the intent of purifying my protein , however due to column...

06 July 2017 4,494 7 View

Has anyone tried to add IPTG to auto inducing media like ZYM? Did you notice an increase in protien expression or is it pointless?

I express using BL21 DE3 e-coli cells and use the ZYM 5052 media. Some colleagues who also use this media have suggested adding IPTG to increase the protein expression. Would that make any sense...

06 July 2017 6,416 3 View

Effect of 1000 folds antibiotic addition to e. coli culture media?

I usually add 100ug/ml of kanamycin to my ZYM culture media. I accidently in a moment of fatigue added the entire 100mg/ml stock to one of my batches! What effect will this have on the cell growth...

07 August 2016 5,051 3 View

My DNA plasmid purification yielded a 260/280 ratio of 2.15. Why?

After following the exact protocol of the NucleoSpin Plasmid purification kit, I measured the concentration to find the 260/280 ratio 2.15! Any idea what could have gone wrong and how can I fix this?

04 May 2016 2,957 4 View

Can I re-induce protein expression after storage at 4 degrees Celsius?

I was expressing protein in E coli cells at 37 degrees and the OD was 1. I had to store the culture over 3 nights, so I stored it at 4 degrees to prevent over expression. After the 3 nights I...

04 May 2016 3,401 4 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

GC-MS retention index prediticon?

Hello experts, Does anyone know any free software about retention index prediction ?

08 August 2024 7,403 2 View

Why does my protein refolded to beta sheet during thermal denaturation analysis?

Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...

06 August 2024 1,989 3 View

How to understand this crystallographic phenomenon of low temperature crystals in zeolite?

During low-temperature testing, new diffraction peaks that appear could be indicative of several phenomena. In one of our tests, we observed notable new peaks around 40° and 45° in a specific...

06 August 2024 726 3 View

Can anyone provide me with molecular docking softwares/ websites?

Molecular docking software/ websites?

02 August 2024 8,704 7 View

Transfection in HEK293T cells?

Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...

31 July 2024 9,892 4 View

Why Powder XRD is performed after cruhsing a crystal if Single Crystal XRD analysis of the same crystal has been performed already ?

What information we can get from PXRD analysis other than from SCXRD analysis of a crystal ?

30 July 2024 6,261 4 View

How to retain the a GFP tagged gene expression in stable cell line?

Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....

29 July 2024 5,007 0 View

How to know the b axis of a single crystal?

How to apply magnetic field parallel to b axis of any crystal

29 July 2024 4,083 2 View

I am working on my Master's thesis on the biogeography of the genus Ruagea and I would like to ask, could someone help me to check whether my result?

I created a file with my outgroup and ingroup species using Beauti, ran it in BEAST, viewed it in Tracer, and then used TreeAnnotator to create a file that I imported into RASP. Could someone...

28 July 2024 2,979 1 View