Dear all, I need to prepare mucin discs following reported procedure (mucin=250 mg, compression force = 10 tonnes for 30 seconds), but I am not getting disc rather getting aggregates.
can I add any moistening/binding agent??? If yes than how much???
One of my friends, every night after falling asleep, his mouth would make a creaking sound after chewing something. This teeth grinding condition would continue until he woke up, and he didn't...
08 August 2024 1,366 5 View
Hello all, I wanted to know, can I use galaxy (USA, Europe or Australia) platform for analyzing the shotgun data, and can it be used for publication purpose as well? Thanks :)
06 August 2024 6,610 4 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
Nothing
01 August 2024 755 1 View
Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...
31 July 2024 9,892 4 View
we have few papers ready for submission, and we need one co-author for each article who can pay article fee. Interested authors may text here or contact me on my following email id [email protected]
29 July 2024 6,626 0 View
In a research, lesion diameter due to a disease has a CV below 10%. However, in an another parameter, disease inhibition percentage (compared to control), a value derived from lesion diameter...
27 July 2024 201 0 View
Hi, can someone please help me with their expertise. To analyse a dataset with 2 IV´s (2 levels each) and 1 DV (time - measured at 4 points, i. e. 4 DV?) I found the two-way repeated measures...
24 July 2024 3,284 6 View
Hello everyone! Someone working with Crispr Cas9. I have a question. Is it possible to edit a gene that is present on a plasmid (in this case with a low copy number)? In this case, is it possible...
22 July 2024 7,073 2 View
How to determine tannin content in fodder pellets?
22 July 2024 8,163 3 View
I have a question regarding the feasibility of using the MTT assay to measure the cytotoxic effects of Snail Mucin on E. coli and Pseudomonas. If this is indeed possible, what is the appropriate...
10 March 2024 9,935 3 View
I am trying to find out phage adsorption varying mucin concentration.
18 May 2023 2,544 2 View
Background Info to experimental set-up: Mutated Mucin-1 is found to be aberrantly expressed in diverse cancers and has been detected in certain mature B-cell malignancies such as Multiple Myeloma...
23 December 2022 5,578 0 View
Both drug and mucin are poorly soluble in buffer. I obtained alternating endothermic and exothermic peaks. Anyone knows why there are alternating peaks?
23 October 2022 1,907 1 View
I would like to determine mucin levels in HT29 cells using alcian blue. Unfortunately alcian blue stains everything blue and I cannot determine the mucin levels. Is there a standard protocol that...
30 March 2022 6,560 2 View
Hi, I want to know "how can I filter / remove the blood and mucin from the media containing bacteria, mucin, and blood". The purpose of my research is to analyze the microbial profile in the...
21 October 2021 1,312 0 View
Dear All, We supplemented a diet containing complex carbohydrates to the chicken and found a shift in microbiota composition and increased propionate and butyrate production in the caecal digesta....
18 October 2021 8,692 0 View
Hi, I use PAS staining to demonstrate the presence of neutral mucins in cell cultures, I was wondering whether anyone had come across an extraction and quantification protocol as this would be...
26 July 2021 480 1 View
Are you aware of any publication on mucinous cystic neoplasm of pancreas where the serum estrogen levels where measured? I am aware that many express ovarian tissue (struma ovarii). I keep getting...
03 June 2021 3,382 3 View
Dear all, I have a question regarding the most appropriate fit of a standard curve in an enzyme-linked lectin assay set-up to quantify the amount of mucin secretion in conjunctival explant...
03 January 2021 1,417 6 View