Dear Victus Kordorwu

ITC gives a signal from any chemical/physical phenomena causing heat flow. Thus, in your case, you probably see peaks from the interaction between drugs and mucin, and in some injections, you see overlaying signal from a dispersion/dissolution of a bunch of sticky molecules from injecting syringe. Because the latter is random and the concentrations of dissolved molecules during the experiment are not defined, you cannot use it for any calculation. Instead, you can either lower the concentrations to those below the solubility limit or find a co-solute to dissolve molecules of interest (e.g., a low percentage of DMSO).