Problems with Alcian Blue Staining of Mucins in HT29 cells?

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How to avoid the high background of PBS (the control of solvent) during Nanoparticle Tracking Analysis (NTA) of exosomes?

Dear colleagues, Has anyone of you ever experienced difficulties with the high background of the solvent-PBS (used to suspend the exosomes after isolation by ultracentrifugation) during NTA...

09 January 2024 7,735 3 View

Hi, where can I find the PMMA CIF file?

I need a basic CIF file of PMMA with their different crystalographic coordinates in the aim to modyfing it in Gaussian :)

25 May 2023 2,983 1 View

When should the growth factors FGF and EGF be added to the culture medium?

Dear Colleagues, I am working with glioma cancer stem cells cultured in suspension as the spheroids. Generally, I used to prepare fresh, complete culture medium just before using to the cells - I...

29 January 2022 2,144 5 View

Nothing on the plot in EEGLab- what could be the reason?

Hello, I have 20 channels EEG data in txt file. I imported it to Matlab and saved as a numeric matrix. When I import it into EEGLab and open a scroll plot there's nothing on the plot. I attach a...

08 June 2020 3,304 9 View

Removal of the free Cy5 dye?

Hello everyone, I was planning to run a FRET experiment to characterize in more detail how my protein of interest inserts the membrane (based on a simple membrane mimicing vesicle model). I was...

29 July 2018 641 2 View

Has anyone done research in subject of using FME for GIS analysis for EIA reports?

I'm working on my MSc project and am searching academic papers on the subject. The title of my research at the moment is "Developing processes to standardise baseline report of the impacts of a...

29 December 2017 8,922 1 View

Has anyone done research in subject of using FME for GIS analysis for EIA reports?

I'm working on my MSc project and am searching academic papers on the subject. The title of my research at the moment is "Developing processes to standardise baseline report of the impacts of a...

29 December 2017 5,374 3 View

How to change residue label in Sparky NMR?

Hello, It's a Sparky beginner. I wanted to make a nice picture with protein assignment but for all assigned residues I have the name format as ex. L166N-HN and I want to avoid "N-HN". I have found...

08 March 2016 7,657 5 View

Currently, we observe an increase in global challenges for energy markets. What do you think about The European Union energy policy?

Currently, we observe an increase in global challenges for energy markets. The European Union is of the opinion that the only way to deal with these challenges is more Europeanized energy policy....

24 March 2015 6,442 14 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

Do I need specific antibodies to stain for a fusion protein?

I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...

07 August 2024 5,338 2 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How to determine positive-stained cells in FACS? Use isotype or unstained control?

To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...

06 August 2024 6,728 6 View

Can I multiplex a mouse monoclonal and rat primary for IHC (mouse brain tissue)?

Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...

06 August 2024 9,710 1 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

How to use Density Functional Theory to calculate carrier mobilities of solid system?

Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...

04 August 2024 8,894 1 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

Senescence-associated beta galactosidase staining is False Positive in control group?

Hi guys If anyone is currently working on aging cells, you guys would like to give me some advice. I'm testing against biomarker (SA-beta-Gal), I encountered a false positive in the control group...

02 August 2024 6,735 1 View

How can I get my Granzyme B flow cytometry stain to be consistent?

I have used PE and PE-Dazzle 594 fluorochromes and have managed to get NK cells to properly show GranzymeB expression after 4 hr PMA/ionomycin stimulaton, but for some reason my CD8 cells in the...

01 August 2024 7,677 2 View

Subhash C. Juneja

May be, you can read this enclosed standard protocol, that may or may not help you, good luck

http://www.ihcworld.com/_protocols/special_stains/alcian_blue.htm

Malcolm Nobre

Hello Sylwia Meczynska

Please refer to the research article attached below. It may be helpful.

Article Gastrointestinal Cell Lines Form Polarized Epithelia with an...

Best.