I don't think it's appropriate for your experimental purpose/design. While MTT Assay is a commonly used assay to assess cell viability and cytotoxic effects in mammalian cells, it may not be suitable for bacteria like E. coli and Pseudomonas, as they do not undergo the same metabolic processes as eukaryotic cells.

For bacteria, alternative methods such as the colony-forming unit (CFU) assay, turbidity measurements, or specific staining techniques are more appropriate to assess viability and cytotoxicity.

Steffany lacara Sulit As bacteria lack the necessary cellular components, such as mitochondria, which are essential for the MTT assay, I suggest you alternative method such as CFU counting, disk diffusion assays that sounds more appropriate for your project. If you have any question please do not hesitate to ask me.

Hi Steffany lacara Sulit!

I saw bacterial contamination may be coloured more them cell culture. There may be two problem which need be examine:

- if your bacterial culture doesn't product formazan from MTT that involve zero result;

- bacterial cells may have too weak adhesion that involve high variation in result.

Good luck!