How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

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03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

How to avoid ulcers in s.c. injected tumors ?

Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...

01 March 2021 9,938 3 View

What is the best method to evaluate and measure non-circulating estrogen levels in mice ?

Could anyone suggest me the best procedure to measure the circulating estrogen levels in mice ? I came across the general methods where it can be measured in serum or urine but a detail suggestion...

22 February 2021 4,352 2 View

How to get the heading of real-time credit card dataset ?

I am working with the Credit card dataset. I am aware of the fact that the Banks and financial institutions do not share the dataset with third-party companies or researchers. I just need to know...

18 February 2021 9,186 3 View

Can we help fellow mathematicians get free ?

https://terrytao.wordpress.com/2021/02/08/a-statement-from-mathematics-department-faculty-at-stanford-and-mit/ Terry Tao blogged about this unfortunate event. Kindly share among Mathematical...

08 February 2021 1,055 3 View

Freeze/Thaw of LS174t cells?

Hello World, I have been working with LS174t. Complete Growth Media- 10% FBS/EMEM. ATCC says to freeze with 5% DMSO/CGM. when I defrost my vials, they are not attaching to flask. vials put into...

01 February 2021 8,723 2 View

RNA extraction from exocrine pancreas?

Hi everyone ! Does anyone have any experience in extracting RNA from exocrine pancreas (which is very high in RNAse)? The pancreas is frozen tissue. I wonder if RNAse is also high in the nucleus,...

31 January 2021 390 2 View

Can I use video interview excerpts from others in public domain (YouTube, Vimeo, etc) for my empirical analysis?

Dear community, I am currently writing my master's dissertation. As a part of the method, I have to include views (interview) of experts for the empirical analysis. The problem is I have not...

31 January 2021 3,438 3 View

I get tumor tissue from the patients after the surgery. How to isolate the top layer of cells from a tumor?

I work on gastric cancer. I want to separate the top layer of tumor cells from the lower layers.

25 January 2021 6,663 1 View

Is aligning mitochondrial and nuclear sequences inconsequential?

Probably a silly question but I am a complete novice in molecular biology. I have decided to attempt a phylogenetic analysis. I have managed to create a phylogenetic tree but want to confirm...

19 January 2021 5,895 2 View

How to estimate the standard deviation of the carbom emission of a company producing many different products?

I am aware of the portfolio theory in general but want to know of a journal or author especialized in this issue.

14 January 2021 7,566 2 View