What is the best Perception instrument that I could use for Student Perception towards a subject?

I am starting to write my thesis for Graduate School and I am struggling to find appropriate instrument regarding student perception instrument. The perceptions to be discussed are (a) interest...

17 January 2021 9,218 3 View

What medical specialties and sub-specialties should be included in a history of medicine bibliography?

I'm in the final stages of compiling a history of medicine bibliography. It will be my second reference work to be published by Jenny Stanford Publishing (my first was "The Nanotechnology...

06 December 2020 3,825 3 View

Is there any scientific evidence that there has been an overarching moral decline among (pre)teen students in the last, say, 10 years?

I hear this type of claim bandied about in certain education circles--namely, by k-12 practitioners themselves--that students' behavior, respectfulness, etc. has all but disappeared. I know that...

15 January 2020 455 2 View

I would like to conduct a research on the Impact of Facebook usage to the Character Building of Grade 10. What question/s do you suggest?

The study will be conducted to grade 10 students in the Philippines

17 July 2019 2,788 3 View

How extract information of molecular chains from .xtc file (gromacs)?

I barely discover Gromacs and I have to use it to study trajectory results, so I wanted to know if it was possible from an xtc file to generate several xtc files, one for each chain of the...

25 March 2019 2,945 5 View

What is the rational basis of the moral obligation of nonviolent noncooperation with and/or resistance to injustice?

At the foundation of principled nonviolence is the moral obligation of noncooperation with and/or resistance to injustice, oppression, tyranny, etc. I am interested in the various schools of...

28 February 2018 3,393 6 View

No title

23 October 2016 5,023 3 View

Due to the drastic price reduction of PV modules, do you think high concentration PV systems are still cost-effective?

The price of PVmodule in about 15 years is 1/10, whereas the price of PV systems is halved [1], it implies that the impact of PV modules on the cost of the whole systems is decreasing more and...

08 November 2013 5,020 4 View

Does anyone have experience with XLSTAT Tukey test (one-way ANOVA results)?

Any reason why there is inconsistency between significant table (contrast) and grouping table (category) in the Tukey test?

02 September 2013 1,666 8 View

Is the reactivity of a functional group affected by the type of molecule that carries it?

Is there a possibility to predict or estimate the reactivity of an epoxy functional group, by means of a comparison whether it is bond to aromatic or aliphatic molecules, respectively?

30 August 2013 8,831 10 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

How to accurately quantify chitin?

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...

01 March 2021 1,843 2 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

Markers of mitochondrial activity in FFPE tissues?

Hello everybody, Does someone have any experience with assessing/extimating mitochondrial activity on FFPE tissues? Precisely, no TOM20, ATP5B, etc: we are trying to get an approximative idea of...

24 February 2021 4,618 4 View

Can a Golgi-stained dendritic spines picture processed by Image J be directly used for publication?

24 February 2021 3,348 2 View

Is there an appropriate CD8 antibody for IHC-P for staining human breast cancer tissues?

Hello, We are planning on staining Formalin-fixed paraffin breast cancer tissue sections (FFPE) with CD8 Ab via immunofluorescence (Tissue IF). Based on our existing protocol and imaging...

24 February 2021 9,578 4 View