Hi all
I am searching for mouse cell lines that have endogenous IGF-1R expression.
Any suggestions would be much appreciated.
Hi all, I am not really good at stats and I have a question to ask; I had two study groups of mice, one treatment and one non-treatment, with 6 mice in each group. I used student's t-test to...
06 July 2018 2,572 4 View
Hi all, I want to study effect of IGF-1 on activation of IGF-1R in mouse by Western blotting. I have come across a few papers where people injected IGF-1 via the inferior vena cava, which I am...
05 June 2018 7,395 0 View
Hi all, Does anyone has any experience successfully detecting protein expression for insulin receptor (IR) and phospho-IR (p-IR) in mouse cell lines by Western blotting? I have tried using 3T3-L1...
04 May 2018 7,348 1 View
I have two groups of mice (n= 6 per group), treatment and control. I am measuring tumour size of these mice every day for 3 weeks. The size will be averaged in each group and I am plotting it over...
11 December 2017 432 4 View
Hi all I am interested in studying on-target effect of a drug a using mouse tail tissues by Western blotting. I cannot find a published literature describing how to homogenise mouse tails...
09 October 2017 8,641 3 View
Hi all I used NP40 lysis buffer for HeLa cells and HEK293T cells and used the whole cell lysates to do Western blot. The main purpose was to determine what cell line express endogenous IGF-1R...
08 September 2016 3,741 5 View
Hi all I am having trouble with detecting phospho-IGF-1R by Western blot. I am trying to detect both the total IGF-1R beta and phosphorylate IGF-1R in MCF-7 and HEK293 by IP and Western blot. I...
07 August 2016 9,560 7 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
I am currently looking for a questionnaire that relates with my study which is The Effectiveness of Organizational Culture towards employee retention rates
03 August 2024 3,505 6 View
I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...
03 August 2024 3,851 1 View