Test to get statistical significant difference with unequal sample sizes?

Hi all, I am not really good at stats and I have a question to ask; I had two study groups of mice, one treatment and one non-treatment, with 6 mice in each group. I used student's t-test to...

06 July 2018 2,511 4 View

I.P injection of IGF-1 in mice?

Hi all, I want to study effect of IGF-1 on activation of IGF-1R in mouse by Western blotting. I have come across a few papers where people injected IGF-1 via the inferior vena cava, which I am...

05 June 2018 7,344 0 View

Insulin receptor in mouse cell lines?

Hi all, Does anyone has any experience successfully detecting protein expression for insulin receptor (IR) and phospho-IR (p-IR) in mouse cell lines by Western blotting? I have tried using 3T3-L1...

04 May 2018 7,300 1 View

What kind of statistical test to use?

I have two groups of mice (n= 6 per group), treatment and control. I am measuring tumour size of these mice every day for 3 weeks. The size will be averaged in each group and I am plotting it over...

11 December 2017 377 4 View

How to do Western blotting with mouse tails?

Hi all I am interested in studying on-target effect of a drug a using mouse tail tissues by Western blotting. I cannot find a published literature describing how to homogenise mouse tails...

09 October 2017 8,551 3 View

Why band in Western blot appears bit lower than expected?

Hi all I used NP40 lysis buffer for HeLa cells and HEK293T cells and used the whole cell lysates to do Western blot. The main purpose was to determine what cell line express endogenous IGF-1R...

08 September 2016 3,683 5 View

How to detect IGF-1R phosphorylation by western blot?

Hi all I am having trouble with detecting phospho-IGF-1R by Western blot. I am trying to detect both the total IGF-1R beta and phosphorylate IGF-1R in MCF-7 and HEK293 by IP and Western blot. I...

07 August 2016 9,495 7 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

What is the ideal efficiency for a qPCR?

hello everyone, I need to do standard curves for my qPCR, what is the ideal efficiency range? I tried a primer (Mglu2 receptor) that gave an efficiency of 90.2%. Is it accepted?

28 February 2021 1,254 3 View

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28 February 2021 9,936 2 View