Test to get statistical significant difference with unequal sample sizes?

Hi all, I am not really good at stats and I have a question to ask; I had two study groups of mice, one treatment and one non-treatment, with 6 mice in each group. I used student's t-test to...

06 July 2018 2,511 4 View

I.P injection of IGF-1 in mice?

Hi all, I want to study effect of IGF-1 on activation of IGF-1R in mouse by Western blotting. I have come across a few papers where people injected IGF-1 via the inferior vena cava, which I am...

05 June 2018 7,344 0 View

Insulin receptor in mouse cell lines?

Hi all, Does anyone has any experience successfully detecting protein expression for insulin receptor (IR) and phospho-IR (p-IR) in mouse cell lines by Western blotting? I have tried using 3T3-L1...

04 May 2018 7,300 1 View

What kind of statistical test to use?

I have two groups of mice (n= 6 per group), treatment and control. I am measuring tumour size of these mice every day for 3 weeks. The size will be averaged in each group and I am plotting it over...

11 December 2017 377 4 View

Mouse cell lines that express IGF-1R?

08 September 2016 9,529 0 View

Why band in Western blot appears bit lower than expected?

Hi all I used NP40 lysis buffer for HeLa cells and HEK293T cells and used the whole cell lysates to do Western blot. The main purpose was to determine what cell line express endogenous IGF-1R...

08 September 2016 3,683 5 View

How to detect IGF-1R phosphorylation by western blot?

Hi all I am having trouble with detecting phospho-IGF-1R by Western blot. I am trying to detect both the total IGF-1R beta and phosphorylate IGF-1R in MCF-7 and HEK293 by IP and Western blot. I...

07 August 2016 9,495 7 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Will a 10xHis-tag prevent anti-MBP from binding to MBP?

I have a protein with a 10xHis-MBP tag on the N-terminus. I'm fairly certain I am expressing my protein, but I'm not getting a signal on my western blot. Is there a possibility that the 10xHis tag...

25 February 2021 8,404 3 View

Looking for genomic DNA extraction kits from mouse tissue (centrifuge-based, not spin-column)?

Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...

25 February 2021 155 2 View

How do I choose contrasts in SPM8?

I am learning how to use SPM for PET and PET-MR analysis, but I haven't found any great resources that explain how contrasts work. I have a series of PET images where our hypothesis argues that...

24 February 2021 1,534 2 View