Yinan Yuan

8 Questions 8 Answers 0 Followers

Questions related from Yinan Yuan

Test to get statistical significant difference with unequal sample sizes?

Hi all, I am not really good at stats and I have a question to ask; I had two study groups of mice, one treatment and one non-treatment, with 6 mice in each group. I used student's t-test to...

07 July 2018 2,538 4 View

I.P injection of IGF-1 in mice?

Hi all, I want to study effect of IGF-1 on activation of IGF-1R in mouse by Western blotting. I have come across a few papers where people injected IGF-1 via the inferior vena cava, which I am...

06 June 2018 7,370 0 View

Insulin receptor in mouse cell lines?

Hi all, Does anyone has any experience successfully detecting protein expression for insulin receptor (IR) and phospho-IR (p-IR) in mouse cell lines by Western blotting? I have tried using 3T3-L1...

05 May 2018 7,326 1 View

What kind of statistical test to use?

I have two groups of mice (n= 6 per group), treatment and control. I am measuring tumour size of these mice every day for 3 weeks. The size will be averaged in each group and I am plotting it over...

12 December 2017 406 4 View

How to do Western blotting with mouse tails?

Hi all I am interested in studying on-target effect of a drug a using mouse tail tissues by Western blotting. I cannot find a published literature describing how to homogenise mouse tails...

10 October 2017 8,605 3 View

Why band in Western blot appears bit lower than expected?

Hi all I used NP40 lysis buffer for HeLa cells and HEK293T cells and used the whole cell lysates to do Western blot. The main purpose was to determine what cell line express endogenous IGF-1R...

09 September 2016 3,709 5 View

Mouse cell lines that express IGF-1R?

Hi all I am searching for mouse cell lines that have endogenous IGF-1R expression. Any suggestions would be much appreciated.

09 September 2016 9,556 0 View

How to detect IGF-1R phosphorylation by western blot?

Hi all I am having trouble with detecting phospho-IGF-1R by Western blot. I am trying to detect both the total IGF-1R beta and phosphorylate IGF-1R in MCF-7 and HEK293 by IP and Western blot. I...

08 August 2016 9,523 7 View