Hi all,
I want to study effect of IGF-1 on activation of IGF-1R in mouse by Western blotting. I have come across a few papers where people injected IGF-1 via the inferior vena cava, which I am not exactly sure how to do. Thus, I am wondering if anyone has I.P injected and looked at phosphorylated status of IGF-1R in vivo.
Cheers