Mitotic index calculation protocol?

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How to differentiate cytoplasm to mitochondrial ROS generation ?

Need a protocol to define ROS generation in different cellular compartments such as cytoplasm and mitochondria. Also need suggestion of any particular inhibitor of ROS for respective compartments

02 March 2017 2,363 4 View

Need help regarding Non-toxic solvent for cell based studies?

In therapeutic analysis for in vitro cell based assays, solubility of the chemical molecules is the most important problem. For water insoluble compounds we generally prefer DMSO (Dimethyl...

07 August 2016 4,248 2 View

Is there any technique available to enrich cancer stem cell population?

I came across a sound number of techniques regarding analytical approach on cancer stem cells or cancer initiating cells (CSC). But I could not able to find out any standardized method to increase...

07 August 2016 10,047 6 View

Is there any standardized protocol available for making 3D cancer cell spheroid micro-environment preparation?

I need to work on in vitro tumor model that will some what similar to the natural tumor. In the model to develop, macrophage (M2 phage) is very crucial including other cells that generally reside...

07 August 2015 2,044 5 View

Does phenol red affect MTT solution ?

We generally use the complete media containing phenol red to seed the cells in multiwell plate.Then after 24 h of culture we treat the cells with the drug containing media containing the drug and...

31 December 2014 2,721 10 View

Is serum free media required for peptide drug treatment?

For peptide treatment in cell culture, which should be the preferred method - with serum or without? Does peptide bind to albumin in serum? Does serum (FBS) contain any proteases? If we will treat...

07 August 2014 6,682 5 View

Can anybody suggest an in-vitro method to analyse the microtubule depolymerisation?

To understand the action of some microtubule target agents. Can anybody help me with a good protocol or work design to analyse depolymerisation of microtubule

07 August 2014 5,721 4 View

Can anyone help regarding immuno-localisation of p53 and p21?

We have tried to localize the p53 and p21 in MCF-7 cells after treatment of drug. We found that p53 located around the nucleus (hoechst stained) but very rare inside signals. Again in negative...

07 August 2014 8,482 7 View

Which online tool will I use for small molecule screening?

Can anybody help me regarding a small molecular screening tool to analyse different lead molecules and how to designing a new one

07 August 2014 9,055 2 View

How does DNA kill the cancer cells ?

I have a dsDNA sequence that contains 14 nucleotides which inserted into cell through nanoparticle and shows good cytotoxicity. Also 6 sequences repeats at two end but in reverse order and two...

06 July 2014 6,409 3 View

What are the emerging trends and opportunities for STEM education and careers in the online space?

10 August 2024 507 0 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is this a facetotecta nauplius?

This larva was captured using a plankton net in the Persian Gulf during the summer. I believe it may be a Facetotecta nauplius.

08 August 2024 3,746 4 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View