How to differentiate cytoplasm to mitochondrial ROS generation ?

02 March 2017 2,242 4 View

Need help regarding Non-toxic solvent for cell based studies?

07 August 2016 4,115 2 View

Is there any technique available to enrich cancer stem cell population?

I came across a sound number of techniques regarding analytical approach on cancer stem cells or cancer initiating cells (CSC). But I could not able to find out any standardized method to increase...

07 August 2016 9,902 6 View

Is there any standardized protocol available for making 3D cancer cell spheroid micro-environment preparation?

I need to work on in vitro tumor model that will some what similar to the natural tumor. In the model to develop, macrophage (M2 phage) is very crucial including other cells that generally reside...

07 August 2015 1,922 5 View

Does phenol red affect MTT solution ?

We generally use the complete media containing phenol red to seed the cells in multiwell plate.Then after 24 h of culture we treat the cells with the drug containing media containing the drug and...

31 December 2014 2,425 10 View

Is serum free media required for peptide drug treatment?

For peptide treatment in cell culture, which should be the preferred method - with serum or without? Does peptide bind to albumin in serum? Does serum (FBS) contain any proteases? If we will treat...

07 August 2014 6,567 5 View

Can anyone help regarding immuno-localisation of p53 and p21?

We have tried to localize the p53 and p21 in MCF-7 cells after treatment of drug. We found that p53 located around the nucleus (hoechst stained) but very rare inside signals. Again in negative...

07 August 2014 8,350 7 View

Which online tool will I use for small molecule screening?

Can anybody help me regarding a small molecular screening tool to analyse different lead molecules and how to designing a new one

07 August 2014 8,922 2 View

How does DNA kill the cancer cells ?

I have a dsDNA sequence that contains 14 nucleotides which inserted into cell through nanoparticle and shows good cytotoxicity. Also 6 sequences repeats at two end but in reverse order and two...

06 July 2014 6,291 3 View

Can any drug which able to increase ROS in cancer cell will have anticancer effect ?

06 July 2014 6,395 11 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

Why are the velocities so high in my Boussinesq approximation Fluent simulation?

Hello, I have a mechanism where air rises due to convective flows. In this simulation, nothing is driving the air except this. I understand that the Boussinesq approximation only works for small...

02 March 2021 4,910 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

What is the characteristic length to determine the Reynolds number for the scattered turbulent fluid flow?

Dear all, I just saw this example in FB on the difference between laminar flow and turbulent flow. The flow from a pipe hits the sink and scattered. The flow from the pipe is characterized as...

02 March 2021 1,999 5 View