15 Questions 18 Answers 0 Followers
Questions related from Saswat Mohapatra
Need a protocol to define ROS generation in different cellular compartments such as cytoplasm and mitochondria. Also need suggestion of any particular inhibitor of ROS for respective compartments
03 March 2017 2,314 4 View
In therapeutic analysis for in vitro cell based assays, solubility of the chemical molecules is the most important problem. For water insoluble compounds we generally prefer DMSO (Dimethyl...
08 August 2016 4,192 2 View
I came across a sound number of techniques regarding analytical approach on cancer stem cells or cancer initiating cells (CSC). But I could not able to find out any standardized method to increase...
08 August 2016 9,995 6 View
I need to work on in vitro tumor model that will some what similar to the natural tumor. In the model to develop, macrophage (M2 phage) is very crucial including other cells that generally reside...
08 August 2015 2,001 5 View
We generally use the complete media containing phenol red to seed the cells in multiwell plate.Then after 24 h of culture we treat the cells with the drug containing media containing the drug and...
01 January 2015 2,662 10 View
To understand the action of some microtubule target agents. Can anybody help me with a good protocol or work design to analyse depolymerisation of microtubule
08 August 2014 5,672 4 View
For peptide treatment in cell culture, which should be the preferred method - with serum or without? Does peptide bind to albumin in serum? Does serum (FBS) contain any proteases? If we will treat...
08 August 2014 6,643 5 View
We have tried to localize the p53 and p21 in MCF-7 cells after treatment of drug. We found that p53 located around the nucleus (hoechst stained) but very rare inside signals. Again in negative...
08 August 2014 8,437 7 View
Can anybody help me regarding a small molecular screening tool to analyse different lead molecules and how to designing a new one
08 August 2014 9,010 2 View
I have a dsDNA sequence that contains 14 nucleotides which inserted into cell through nanoparticle and shows good cytotoxicity. Also 6 sequences repeats at two end but in reverse order and two...
07 July 2014 6,365 3 View
One of our drugs is showing an anticancer effect. It has been found to show G1 phase arrest, apoptosis and microtubule depolymerisation. Besides significant ROS generation detect by ROS. Can...
07 July 2014 6,492 11 View
One of our drugs is showing positive Annexin V -Pi and good MTT result that showing cytotoxic activity but there is no immunofluorescent detection of p53 and p21 was found. We have also found...
02 February 2014 1,891 5 View
Hi,can any body tell me the easiest cell culture protocol to calculate metaphase / anaphase ratio?
02 February 2014 1,574 2 View
The Golden Ratio is generally used in mathematics and arts science based on Fibonacci number. Definitely we have used this technique after the great mathematician, i.e. nature. You can see that...
07 July 2013 6,720 3 View
Dominant genes always need to suppress the recessive genes in a definite way.But which type of regulation they prefer? Transcriptional? Translational? If transcriptional then does the LCR do the...
05 May 2013 7,356 6 View
