Why different protein activity from multiple codons for the same amino acid???

hii all i'm asking why multiple codons code for the same amino acid but different protein activity ? please attach papers or reviews thank you all zaynab

11 December 2018 2,673 5 View

"kit of pyruvate concentration" for serum samples are good or suitable for cell line suspension??

Have a nice day colleague i'm going to determine pyruvate concentration from cell line suspension (before and after treatment) for different duration times using Elabscience pyruvate assay kit...

09 October 2018 1,820 3 View

Is there protocol for restraining slids of immunohistchemistry?

04 May 2018 397 2 View

No title

03 April 2018 3,121 4 View

Iraq cancer registry 1990-2000??

have a nice day i'm very interested to study the statistic and gradual increase of cancer incidence in iraq specially between 1999-2000 year i search and couldn't find something i can depend just...

31 December 2017 6,408 1 View

How can i prepare skimmed milk?

03 April 2016 3,402 11 View

I have an extra band ... why?

02 March 2016 4,062 3 View

Any protocol for protein extraction?

02 March 2016 6,231 7 View

Is their any differences between ECL western blotting apparatus and gel band fluorescent detection apparatus?

31 December 2015 2,809 2 View

Why are there no microRNAs found on Y chromosome?

While reading a lot of articles about biogenesis and importance of microRNAs in diagnosis of diseases and cancer and the ability of using microRNAs in treatment ... in this time i faced that there...

11 December 2015 2,596 5 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

No title

02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View