Have a nice day colleague
i'm going to determine pyruvate concentration from cell line suspension (before and after treatment) for different duration times using Elabscience pyruvate assay kit ELISA assay reader. the issue i forced the kit recommended using serum/ plasma /tissue samples, could i use it for cell line ??
thanks in advance
greetings
Zaynab
There are 2 matters to consider:
(1) Is the kit sensitive enough? The stated sensitivity is 0.006 μmol/mL. Do you think that you can prepare samples that will have at least this concentration of pyruvate?
(2) Do your samples cause interference? I suspect that a kit that can be used for serum and tissue samples will not be bothered by cell line suspensions, which are cleaner samples than tissue homogenates. Make sure you homogenize the cells well enough to release the pyruvate, and clarify the homogenate by centrifugation, as recommended in the kit instructions.
Zaynab Saad
https://www.abcam.com/pyruvate-assay-kit-ab65342.html
https://www.cellbiolabs.com/sites/default/files/MET-5029-pyruvate-assay-kit.pdf
Best wishes
Sincerely
Prabu
Cell line=Tissue
Beaware of GPT enzyme in the cells, you have to be sure about lack of this enzyme in the cell homogenate. Good luck.
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